在磁性金属氧化物纳米颗粒的生物降解过程中,阿波费里丁蛋白的物理化学变化
Ehsan Rahimi1, Amin Imani2, Donghoon Kim3
1Department of Materials Science and Engineering, Delft University of Technology, Mekelweg 2, 2628 CD Delft, The Netherlands.
ACS applied materials & interfaces
|September 17, 2024
概括
治疗性磁氧化物纳米粒子 (MONPs) 在体内迅速降解,改变阿波费里丁蛋白质并可能造成健康风险. 这项研究揭示了MONPs如何改变apoferritin.
科学领域:
- 纳米医学是一种纳米医学.
- 生物材料科学 生物材料科学
- 蛋白质化学 蛋白质化学
背景情况:
- 治疗性磁氧化物纳米颗粒 (MONPs) 在体内生物降解是它们的有效性和安全性的关键问题.
- 与阿波费里丁等蛋白质的相互作用加速MONP的降解,释放金属离子,并可能改变蛋白质的结构和功能.
- 了解这些相互作用对于预测MONP寿命和减轻相关健康风险至关重要.
研究的目的:
- 为了研究与铁酸盐 (CoFe2O4) MONPs.incubation后的阿波费里丁的结构和组成变化.
- 分析MONP诱导的改变对阿波费里丁的电表面潜力 (ESP) 和整体功能的影响.
- 开发基于蛋白质-纳米粒子相互作用的MONP寿命和毒性的预测方法.
主要方法:
- 化阿波费里丁与铁酸盐 (CoFe2O4) 的MONPs.
- 原子力显微镜 (AFM) 用于评估蛋白质地形和高度的变化.
- 扫描凯尔文探针力显微镜 (SKPFM) 用于绘制电面电位 (ESP) 分布的变化.
主要成果:
- 在48小时的潜伏期内观察到阿波费里丁纳米薄膜拓和ESP的显著变化.
- 典型的阿波费里丁孔消失了,由于纳米粒子组件填充了孔,蛋白质高度增加了.
- 检测到ESP的实质性增加,归因于在apoferritin中形成的异质化学成分和晶体结构.
结论:
- MONP-apoferritin的相互作用导致蛋白质的结构和组成发生显著的改变.
- 这些变化,包括孔填充和ESP变化,影响蛋白质-纳米粒子静电相互作用.
- 该研究提供了一种方法来预测MONP的寿命,并通过了解和预防apoferritin变形来降低毒性.
更多相关视频
15:03Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection
Published on: June 16, 2020
9.2K
08:13Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles
Published on: February 27, 2021
4.5K
相关概念视频
02:49Corrosion
29.5K
The degradation of metals due to natural electrochemical processes is known as corrosion. Rust formation on iron, tarnishing of silver, and the blue-green patina that develops on copper are examples of corrosion. Corrosion involves the oxidation of metals. Sometimes it is protective, such as the oxidation of copper or aluminum, wherein a protective layer of metal oxide or its derivatives forms on the surface, protecting the underlying metal from further oxidation. In other cases, corrosion is...
29.5K
01:24Microbes and Other Elemental Cycles
71
Microbial activity plays a pivotal role in the biogeochemical cycling of iron and manganese, especially at the redox gradients characteristic of stratified aquatic environments. These cycles are driven by microbial transformations between oxidized and reduced forms of the metals, allowing organisms to exploit them for metabolic energy and structural purposes.Iron Cycling Across Redox GradientsIn neutral, oxygen-rich surface waters, iron is predominantly found in its oxidized, insoluble ferric...
71
01:19Acid Mine Drainage
81
Mining activities that disturb sulfide-rich rocks, particularly those containing pyrite (FeS₂), initiate a cascade of geochemical and microbiological processes with serious environmental implications. When exposed to air and water, pyrite undergoes oxidation, releasing sulfate, ultimately forming sulfuric acid and mobilizing heavy metals into surrounding water systems. This phenomenon, known as acid mine drainage (AMD), results in low pH waters laden with toxic elements that threaten...
81