线粒体β-氨合成酶 参与flg22诱导的口腔免疫
Rosario Pantaleno1, Denise Scuffi1, Paula Schiel1
1Instituto de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina.
Plant, cell & environment
|September 17, 2024
概括
线粒体硫化 (H2S) 对植物的口腔免疫非常重要. 缺乏这种H2S来源的植物对病原体的防御能力降低,突出显示H2S.
科学领域:
- 植物生理学 植物生理学
- 植物免疫学 植物免疫学
- 细胞信号传输 细胞信号传输
背景情况:
- 保护细胞调节植物气体交换和防御反应.
- 由病原体相关分子模式 (PAMPs) 触发的口腔免疫包括活性氧物种 (ROS) 和硫化 (H2S).
- 虽然研究了细胞质H2S来源,但线粒体H2S在口腔免疫中的作用仍然不清楚.
研究的目的:
- 为了研究线粒体H2S源,β-cyanoalanine合成酶CAS-C1在鞭毛蛋白 (flg22) 诱导的口腔免疫中的作用.
- 探索线粒体H2S在ROS生产中的参与以及对细菌病原体的敏感性.
主要方法:
- 在Cas-c1突变植物和野生类型中分析口腔闭塞和阿波塑性ROS产生.
- 用线粒体H2S供体 (AP39) 进行治疗,并评估RBOHD依赖的ROS产量.
- 药理上抑制线粒体电子运输链活动及其对口腔关闭的影响.
主要成果:
- 缺乏CAS-C1的植物表现出flg22触发的口腔关闭受损,并减少了可塑性ROS的产生,从而增加了对细菌注射的敏感性.
- 线粒体H2S捐赠者AP39以RBOHD-依赖的方式诱导了口腔关闭.
- 线粒体电子运输链活动的破坏影响flg22诱导的口腔关闭.
结论:
- 由CAS-C1产生的线粒体H2S对于对细菌PAMP的有效口腔免疫是必不可少的.
- 线粒体H2S信号与护卫细胞中RBOHD介导的ROS产生有关.
- 细胞内器官,特别是线粒体,在调节植物口腔信号和免疫反应方面发挥着重要作用.
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