一种基于乳清蛋白分离物和异的乳清细菌 (Lactobacillus rhamnosus) 保护剂:抗压力和潜在机制
Yanghua Yuan1, Xiaoyu Jiang1, Wanbing Li1
1College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
International journal of biological macromolecules
|September 17, 2024
概括
这项研究开发了一种新的喷雾干燥乳清蛋白分离物 (WPI) 和异盐酸 (ISO) 保护剂,以提高益生菌的稳定性. 在各种压力下,WPI-ISO混合物显著提高了Lactobacillus rhamnosus的生存率,提供了一个有前途的食品工业战略.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 益生菌在食品加工过程中面临重大压力,影响其生存能力和有效性.
- 开发有效的保护剂对于保持益生菌的稳定性和功能至关重要.
研究的目的:
- 评估喷雾干燥的乳清蛋白分离物 (WPI) 和异盐酸 (ISO) 作为益生菌的新型保护剂.
- 评估不同WPI-ISO比率对物理性能和Lactobacillus rhamnosus对环境压力的耐受性的影响.
主要方法:
- 对物理性质 (湿度,水活性,湿度,玻璃过渡温度) 进行调查的WPI-ISO比率 (1:0, 2:1, 1:1, 1:2).
- 在使用WPI-ISO保护剂的热,储存和模拟胃肠道条件下评估了Lactobacillus rhamnosus的生存率.
- 在储存90天后测量了可活性的细菌数量.
主要成果:
- WPI-ISO保护剂降低了水分含量和水活性,提高了粉末的稳定性.
- 异盐添加显著改善了L. rhamnosus的抗压能力和环境耐受性.
- 90天后,益生菌的存活率超过9毫克CFU/g,证明了有效的应激保护.
结论:
- WPI-ISO混合物为益生菌提供了协同保护作用.
- 这种新型保护剂改善了益生菌的稳定性和在食品应用中的生存.
- "WPI-ISO战略为增强益生菌产品提供了一个有前途的方法.
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