表面抹布与光学显微镜的整合,用于检测和量化来自不钢表面的细菌细胞
Yuzhen Zhang1, Zili Gao1, Lili He1
1Department of Food Science, University of Massachusetts, Amherst, MA 01003, United States.
Letters in applied microbiology
|September 17, 2024
概括
使用聚氨泡和超声波辅助释放的优化扫描采样实现了在不钢上的Salmonella enterica高回收率. 这种快速方法为环境监测提供了准确的细菌检测和量化.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 分析化学 分析化学
背景情况:
- 片采样对于在表面检测微生物至关重要.
- 优化扫描方法和检测试验是准确结果的关键.
- 不钢表面在各种环境中很常见,需要有效的微生物监测.
研究的目的:
- 为优化从不钢表面回收细菌的抽样采样.
- 评估影响抽样采集和释放光学检测的因素.
- 为了实现细菌细胞的最佳检测和量化.
主要方法:
- 评估的氨基二碳酸湿的聚氨泡抹布,用于温和的收集.
- 在NH4HCO3溶液中利用超声波辅助释放用于微生物恢复.
- 采用光学检测试验进行集成的抽样采样,用于细菌定量.
主要成果:
- 通过使用最佳的擦拭和释放方法,Salmonella enterica的恢复率高达92.71%.
- 鉴定PuritanTM无菌大泡抹布为提供最低的背景噪声和最高的恢复效率.
- 在检测到和理论细胞数 (10^4-10^7 CFU) 之间显示出强烈的线性关系 (r2 = 0.9983).
- 确定了7.2 × 10^4 CFU/100 cm2的检测极限.
- 在2小时内完成了综合方法.
结论:
- 优化片采样和超声波辅助释放为不钢提供了高效的细菌恢复.
- 开发的方法为环境监测提供了准确的量化和低检测极限.
- 这种快速有效的技术在需要微生物表面分析的各种环境中具有广泛的适用性.
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