酵母的体外益生菌特征与其后生菌的抗氧化活性和生物膜抑制能力
Furkan Aydın1, Yeşim Aktepe2, Halil İbrahim Kahve3
1Department of Food Engineering, Faculty of Engineering, Aksaray University, 68100, Aksaray, Turkey. furkanaydin@aksaray.edu.tr.
Current microbiology
|September 17, 2024
概括
来自发酵食品的酵母菌株显示出强大的益生菌潜力和后生菌性质. 选择的菌株表现出优异的抗氧化活性和生物膜抑制,在食品和健康领域提供了有前途的应用.
科学领域:
- 食品微生物学 食品微生物学
- 益生菌和益生菌和益生菌.
- 酵母生物技术 酵母生物技术
背景情况:
- 传统的发酵食品是各种酵母菌株的丰富来源.
- 研究酵母菌株的益生菌潜力和后生菌性质对于健康应用至关重要.
- 抗氧化活性 (AOA) 和生物膜抑制能力 (BIC) 是关键的功能特征.
研究的目的:
- 评估从发酵食品中分离出来的酵母的体外益生菌潜力和后生物特性.
- 为了评估抗氧化活性 (AOA) 和生物膜抑制能力 (BIC) 的选定酵母菌株及其无细胞超体 (CFS).
- 确定优质酵母菌株,以作为益生菌或功能性食品的潜在用途.
主要方法:
- 酵母菌株的分子识别使用起始码子向多态.
- 益生菌特征的体外评估:在模拟的胃/胰腺汁中生存能力,自身聚合,疏水性和AOA.
- 对病原性细菌 (Cronobacter sakazakii,Listeria monocytogenes,Pseudomonas aeruginosa,Staphylococcus aureus) 进行AOA和BIC的CFS评价. 这些细菌可能会导致疾病.
主要成果:
- 一些酵母菌株,包括Kluyveromyces lactis,Saccharomyces cerevisiae,Pichia kudriavzevii,Wickerhamomyces anomalus和Torulaspora delbrueckii,在体外表现出显著的益生菌特性,其表现优于参考菌株Saccharomyces boulardii MYA-796.
- 与活细胞相比,无细胞超水体 (CFS) 显示了增强的AOA,并显示出强烈的BIC,特别是针对Listeria monocytogenes和Staphylococcus aureus.
- 特定的菌株 (K. lactis TC11,S. cerevisiae M33T1-2,P. kudriavzevii S96,W. anomalus OB7Y1,T. delbrueckii KY31) 根据胃存活,自聚和AOA,被确定具有优越的益生菌潜力.
结论:
- 从传统发酵食品中分离出来的酵母菌株具有宝贵的体外益生菌和后益生菌功能.
- 来自某些酵母菌株的CFS表现出强大的抗氧化和抗微生物特性,对关键的食源性病原体有效.
- 已识别的优质酵母菌株有望发展为食品和卫生部门的功能成分.
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