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解剖管泄漏发生的独立于与手术性肠道准备相关的微生物群变化
Sonja Boatman1, Mohammad Haneef Khan1, Nirupa Ganesan1
1Basic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA.
Scientific reports
|September 17, 2024
概括
在小鼠的结肠手术后,肠道准备方法并没有降低肠道泄漏率. 研究人员确定了与泄漏有关的特定细菌,这表明了手术并发症的潜在诊断工具.
科学领域:
- 胃肠病学 胃肠病学
- 手术瘤学手术瘤学
- 微生物组研究 微生物组研究
背景情况:
- 感染性并发症,如解肠流漏 (AL),是肠道手术后的重大风险.
- 目前的做法往往假定通过肠道准备进行肠道消毒可以减少手术后的并发症,但证据有限.
研究的目的:
- 调查机械肠道制备 (MBP) 和口服抗生素 (OA) 在结肠手术后预防AL的疗效.
- 探索饮食对手术结果和AL发展的影响.
- 为了确定与AL相关的微生物标记物.
主要方法:
- 用于结肠外科手术的小鼠模型.
- 给小鼠的是普通的或西方饮食.
- 手术前的干预包括水 (对照),MBP,OA或MBP + OA.
主要成果:
- 干预组之间没有观察到AL率的显著差异.
- 饮食没有明显影响手术结果或AL发病率.
- 虽然肠道微生物组的组成随着治疗而改变,但对AL或相关生物标志物 (便短链脂肪酸,结肠RNA) 没有确定具体影响.
- 特定的细菌系与AL相关,导致从便样本开发了一个预测指数.
结论:
- 在这种小鼠结肠手术模型中,标准的肠道准备策略 (MBP,OA) 无法防止解肠漏洞.
- 饮食因素没有显著影响AL的发病率.
- 开发了一种潜在的基于微生物的AL预测工具,需要进一步验证和机制性调查.
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