一个分子流行病学和Clostridioides difficile的传播分析,使用在一家医院的全基因组测序草案
Taito Miyazaki1,2, Kotaro Aoki3, Tadashi Maeda2
1Infection Control Section, Toho University Omori Medical Center, Tokyo, Japan.
BMC infectious diseases
|September 17, 2024
概括
全基因组测序揭示了在医院中沉默的Clostridioides difficile传播,特别是在患者房间的变化. 这凸显了WGS在感染控制和监测C. difficile传播方面的价值.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
背景情况:
- 产生毒素的Clostridioides difficile在体内传播是一个主要的感染控制挑战.
- 困难菌居住在人类的肠道中,可以通过与医疗人员的密切接触传播.
研究的目的:
- 为了调查C. difficile在单个医疗保健中心内的医院传染模式.
- 使用全基因组测序 (WGS) 分析C. difficile菌株的遗传相关性.
主要方法:
- 在2014年4月至2015年1月间从患者中分离的38种C. difficile菌株的回顾性分析.
- 利用全基因组测序 (WGS) 草案来检查核心基因组中的单核酸多态 (SNP).
- 与患者医院病房和房间转移信息相关联的遗传数据.
主要成果:
- 确定了38个属于11个不同的序列类型 (ST) 的菌株,其中ST81,ST183和ST17是最常见的.
- SNP分析揭示了疑似医院传播 (SNT) 的五个集群,涉及38种菌株中的16种.
- 最大的SNT集群 (ST183-SNT-1) 涉及A,B和K病房的五名患者;经常改变房间和患者入院/出院.
结论:
- 在WGS和SNP分析草案中,在医院病房内发现了沉默的C. difficile传播.
- 频繁的病房变更和重复的入院/出院与传播风险的增加有关.
- 基于WGS的C. difficile监测为感染控制管理提供了宝贵的工具.
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