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Updated: May 8, 2026

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

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用于PCR的活性诊断成分 (ADI):一个微型生物反应器用固定R5-激酶产生dNTP

Anna R Bird1, Elizabeth A H Hall1

  • 1Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.

Biotechnology and bioengineering
|September 18, 2024
PubMed
概括

这项研究介绍了一种新型的酶生物反应器,用于生产脱氧核酸三酸盐 (dNTP),这是核酸放大测试 (NAAT) 的关键试剂. 这项创新旨在通过解决供应链和成本障碍来改善资源有限的环境中的NAAT可访问性.

关键词:
活性诊断成分 (ADI) 的使用.在 dNTP 中使用 dNTP在体外诊断 (IVD)资源较低的地区.微型生物反应器微型生物反应器核酸放大试验 (NAAT) 是指核酸放大试验.

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相关概念视频

PCR01:32

PCR

Overview
PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...