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葡萄甘是一种有前途的异化增强剂,可诱导巨细胞细胞分裂
Bernadette Dian Novita1, Widya Wasiyastuti2, Yudy Tjahjono3
1Department of Pharmacology and Therapy, Faculty of Medicine, Widya Mandala Surabaya Catholic University, Surabaya, Indonesia.
Journal of advanced pharmaceutical technology & research
|September 18, 2024
概括
葡萄甘增强了巨细胞的活性,并增强了对抗耐药结核病的异化的疗效. 这项研究表明,葡萄甘可以改善 fagocytosis 在不影响 isoniazid 的情况下.
科学领域:
- 免疫学和微生物学
- 药理学和药物发现
背景情况:
- 伊索尼亚齐德 (INH) 是结核病 (TB) 的首要治疗方法,但越来越多的抗药性菌株,包括耐INH的*Mycobacterium tuberculosis* (Mtb),需要新的策略.
- 增强巨细胞化是克服INH耐药性的潜在方法.
- 葡萄甘是一种多糖,具有免疫调节特性,可以激活巨细胞.
研究的目的:
- 从*Amorphophallus muelleri*中描述葡萄干,并评估其对巨细胞活动的影响.
- 评估葡萄甘南-INH混合物对INH对MTB的疗效的影响.
- 为了确定葡萄甘是否会影响巨细胞的活力和细胞能力.
主要方法:
- 来自*Amorphophallus muelleri*的葡萄甘 (0.05%-0.2%) 进行了分析.
- 在Mtb菌株H37RV上进行了INH敏感性测试.
- 测量了小鼠巨细胞 (RAW264.7) 的活力 (MTT测定) 和细胞 (乳珠测定).
- 使用紫外线光谱测量来评估化学结构的变化.
主要成果:
- 葡萄甘 (0.05%-0.2%) 显著改善了巨细胞的活力和活性.
- 与单独使用INH相比,葡萄甘南和INH的组合显示了IC50的增加,这表明疗效提高.
- 细胞测定证实了0.05%和0.1%的葡萄康曼南的巨细胞活性升高.
- 葡萄康南没有降低INH的有效性,也没有改变其化学结构.
结论:
- 葡萄甘,特别是较低分子量分数,通过促进巨细胞细胞增生,有望增强异化物对抗INH耐药Mtb的有效性.
- 这些发现挑战了以前关于葡萄康南对药物吸收的影响的假设.
- 需要进一步的研究来确定特定的受体,补充受体是葡萄甘诱导的细胞分裂的潜在媒介.
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