相关实验视频

Updated: Jun 12, 2025

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

构建circRNA介导的ceRNA网络和免疫试验,用于调查COPD的病原性

Ting Yang^1,2, Wenya Xu³, Jie Zhao³

¹Department of General Practice, Hainan Affiliated Hospital of Hainan Medical University, Hainan General Hospital, Haikou, Hainan, China.

Frontiers in genetics

|September 18, 2024

概括

这项研究确定了circDTL-hsa-miR-330-3p-CCL20网络作为慢性阻塞性肺病 (COPD) 的关键机制. 这一发现为COPD治疗提供了新的治疗目标.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
免疫学免疫学免疫学

背景情况:

慢性阻塞性肺病 (COPD) 是全球主要的健康负担,导致大量死亡和残疾.
了解COPD背后的复杂分子机制对于开发有效的治疗方法至关重要.

研究的目的:

阐明COPD病变发生过程中的新型分子标和机制.
在COPD中构建和分析竞争性内源RNA (ceRNA) 网络.

主要方法:

对COPD数据集的差异性基因表达分析 (GSE38974,GSE106986).
使用Cytoscape构建蛋白质-蛋白质相互作用 (PPI),mRNA-miRNA联合表达和ceRNA网络.
使用CIBERSORT和拉索模型对免疫透细胞和枢纽基因进行查.
通过RT-qPCR在COPD细胞模型中CeRNA网络因子表达的体外验证.

主要成果:

确定了852个差异表达的基因,包括7个枢纽基因.
通过网络分析和ROC曲线验证了CCL20作为一个关键的枢纽基因.
构建了circDTL-hsa-miR-330-3p-CCL20 ceRNA网络. 这是一个很好的例子.
证明了CCL20通过化学因子在免疫细胞迁移中的作用,并证实了COPD细胞模型中circDTL,hsa-miR-330-3p和CCL20的改变表达.

结论:

该circDTL-hsa-miR-330-3p-CCL20网络提供了对COPD分子机制的见解.
这个网络代表了开发新型COPD治疗策略的潜在治疗目标.

关键词:

CCL20 CCL20 CCL20 CCL20 CCL20 CCL20 CCL20 CCL20 CCL20 慢性阻塞性肺病慢性阻塞性肺病环DTLTL 的时间具有竞争力的内源性RNA.这就是hsa-miR-330-3p.

更多相关视频

In vivo Evaluation of Mucociliary Clearance in Mice

In vivo Evaluation of Mucociliary Clearance in Mice

Published on: December 18, 2020

Quantification of Circular RNAs Using Digital Droplet PCR

Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

相关概念视频

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation

COPD: Pathogenesis and Clinical Features

COPD: Pathogenesis and Clinical Features

Chronic obstructive pulmonary disease (COPD) is a group of lung conditions that progressively worsen over time, including chronic bronchitis and emphysema. This cluster of diseases collectively leads to a gradual and irreversible decline in lung function over time.
The primary cause for the onset of COPD is cigarette smoking and exposure to air pollution. These hazardous factors initiate a chain reaction within the lungs, resulting in chronic inflammation, damage to the airways, and a...

Chronic Obstructive Pulmonary Disease-IV: Assessement and Diagnostic Studies

Chronic Obstructive Pulmonary Disease-IV: Assessement and Diagnostic Studies

Assessing and diagnosing Chronic Obstructive Pulmonary Disease (COPD) involves a detailed approach that includes a comprehensive review of medical history, physical examination, and a variety of diagnostic tests. This thorough evaluation is essential to ensure an accurate diagnosis and guide effective management strategies.
Medical History

Chronic Obstructive Pulmonary Disease-I: Introduction

Chronic Obstructive Pulmonary Disease-I: Introduction

Chronic Obstructive Pulmonary Disease (COPD) is a long-lasting respiratory condition requiring continuous attention and care. It is a progressive lung disease that leads to breathing challenges due to airflow obstruction. It manifests as persistent respiratory symptoms and restricted airflow resulting from abnormalities in the airways and alveoli, usually due to long-term exposure to harmful particles or gases. COPD mainly consists of two primary conditions: emphysema and chronic bronchitis.