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使用微流体适应通道和多重光显微镜快速识别细菌分离物

Stelios Chatzimichail^1,2, Piers Turner^1,2, Conor Feehily³

¹Department of Physics, University of Oxford, Parks Road, Oxford, OX1 3PJ, UK. stelios.chatzimichail@physics.ox.ac.uk.

|September 18, 2024

概括

适应性通道细菌捕获 (ACBC) 装置从低密度样本中快速捕获和识别细菌病原体. 这项技术可以快速识别物种,并进行抗生素敏感性测试,以改善诊断.

科学领域:

微流体学微流体学
细菌学细菌学是一门学科.
诊断技术诊断技术的使用

背景情况:

传统的细菌捕获和识别方法往往耗时且技术要求很高.
快速检测细菌病原体对于及时的临床干预和疾病控制至关重要.

研究的目的:

为了展示一种新的微流体装置,即适应性通道细菌捕获 (ACBC),用于快速捕获,丰富和识别细菌病原体.
评估ACBC在捕获低密度细菌样本方面的效率及其与物种识别和抗微生物敏感性测试的整合.

主要方法:

使用聚二甲基西洛 (PDMS) 微流体装置,可控制反压,形成细菌捕获区域.
采用多重16SrRNA-光现场杂交 (FISH) 来进行物种识别.
集成的ACBC与使用光成像和卷积神经网络 (CNN) 分类的超快速抗微生物敏感性测试 (AST) 方法.

主要成果:

从度低于1000细胞/毫升的样本中获得近100%的细菌捕获效率.
在一小时内成功识别了七种细菌物种,包括*大肠杆菌*和*金黄色葡萄球菌*,准确度为70-98%.
证明了ACBC芯片作为成像流动细胞计的能力,用于预测大肠杆菌的抗生素敏感性.

结论:

ACBC设备为细菌病原体检测和表征提供了一个快速,高效和低成本的替代方案.
综合系统允许快速识别物种和预测抗生素敏感性,为更快的临床诊断铺平道路.
这项技术有可能显著提高细菌感染诊断的速度和准确性.

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