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Updated: Jun 12, 2025

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Published on: March 24, 2016

使用顶帽光学对IR-MALDESI质谱成像进行绝对量化谷氨

Emily R Bruce¹, Russell R Kibbe¹, Emily C Hector²

¹FTMS Laboratory for Human Health Research, Department of Chemistry, North Carolina State University, Raleigh, North Carolina, USA.

Journal of mass spectrometry : JMS

|September 18, 2024

概括

在红外矩阵辅助激光消化喷射电离 (IR-MALDESI) 中,一种新的衍射光学元件 (DOE) 保持了质谱成像 (MSI) 的定量精度. 这种DOE提供了改进的能量分配,而不会影响像谷氨这样的生物分子的绝对量化.

科学领域:

分析化学分析化学
生物物理化学生物物理化学
频谱学是一种光谱学.

背景情况:

红外矩阵辅助激光脱电喷雾电离 (IR-MALDESI) 用于大气压脱和生物分子的电离.
在IR-MALDESI中的常规光学产生高斯激光轮,导致由于循环能量分布导致邻近组织的不良加热.
引入了一种衍射光学元件 (DOE),以创建顶帽光束形状和具有均能量分布的方形消光点.

研究的目的:

评估 DOE 对 IR-MALDESI 质谱成像 (MSI) 定量能力的影响.
为了比较DOE配备的光学列车与常规光学对定量MSI (qMSI) 的性能.
确定能源部的顶帽光束形状是否为MSI的绝对量化提供了优势.

主要方法:

使用同质谷氨酸 (hGSH) 作为内部标准,对谷氨酸 (GSH) 的绝对量化.
准备一种稳定同位素标记为谷氨 (SIL-GSH) 的稀释序列.
使用IR-MALDESI MSI进行分析,使用传统光学和美国能源部合并的光学列车.

主要成果:

用于GSH量化的校准曲线显示,传统和DOE光学列车之间没有显著差异.
美国能源部配备的系统实现了与传统系统相比的绝对量化.
统计分析证实,这两种光学配置的定量准确性表现相似.

结论:

在IR-MALDESI中,DOE可以有效地用于qMSI,而不会影响绝对量化准确度.
能源部为未来的qMSI应用提供了统一的能量配置和方形的消光点的好处.
这一进步支持DOE用于改进和更精确的定量质谱成像.

关键词:

光光学元件是一种光光学元件.质谱仪成像成像质谱仪成像成像在QMSI中,QMSI是顶帽子 IR-MALDESI 上一个帽子 IR-MALDESI

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