Tn7

Chyden Chang1,2,3, Minh-Duy Phan1,2,3, Mark A Schembri1,2,3

  • 1Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia.

概括

这项研究增强了微型Tn7载体,用于细菌中稳定的染色体基因集成. 新的诱导和构成性促进剂为补充和合成生物学应用提供了精确的控制.

相关概念视频

DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
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Transgenic Organisms00:53

Transgenic Organisms

Overview
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Transgenic Plants02:50

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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