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结核患者的PPE59抗体以及与其他快速生物标志物进行测试时对诊断的潜在用途
Ana Carla de Paulo Mulinari1, Isabela Gama Sardella1, Vania Maria C da Silva2
1Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Microbiologia Celular, Rio de Janeiro, RJ, Brasil.
Memorias do Instituto Oswaldo Cruz
|September 18, 2024
概括
针对PPE59的抗体测试显示,结核病患者的检测率中等. 将PPE59 IgA与其他测试相结合,可显著提高结核病快速检测的灵敏度.
科学领域:
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
- 微生物学 微生物学
背景情况:
- 结核病 (TB) 是一个重大的全球卫生挑战.
- 在BCG菌株中缺少的PPE59蛋白可能会引起结核病患者的幽默免疫反应.
- 需要进一步的研究来了解PPE59在结核免疫力中的作用.
研究的目的:
- 评估结核病患者对PPE59抗原的抗体反应.
- 评估PPE59抗体与其他真菌细菌抗原和唾液涂抹结果的联合诊断潜力.
- 探索PPE59作为结核病潜在生物标志物的实用性.
主要方法:
- 克隆并表达了编码PPE59.9的rv3429基因.
- 在212个结核病患者和对照组的血清样本中使用ELISA测量了针对PPE59的IgG,IgM和IgA抗体.
- 分析结果与之前的IgG数据相结合,与其他真菌细菌抗原和唾液涂抹微生物学对比.
主要成果:
- 针对PPE59的IgA的敏感度为42.7%,特异性为97%,表现优于IgG和IgM.
- 将PPE59IgA与16kDaIgG结合,以及唾液涂抹结果,使灵敏度提高到86.5%,特异性为88.9%.
- 阳性IgA反应与成像检测结果相关,表明结核病的概率很高.
结论:
- 在结核病患者中,PPE59 IgA显示中度阳性.
- 结合测定 (PPE59 IgA/16kDa IgG/Sputum Smear) 提供了显著提高结核病检测的灵敏度.
- 这种多标志物方法是一个有希望的,尽管不完美的,用于快速诊断结核病的工具,特别是在资源有限的环境中.
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