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相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy01:26

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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相关实验视频

Updated: Jun 12, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
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可调节的超快速差异位移全息技术.

T M Hutchinson1, G Righi1, P M Celliers1

  • 1Lawrence Livermore National Laboratory, Livermore, California 94550, USA.

The Review of scientific instruments
|September 18, 2024
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概括

一种新的数字全息显微镜诊断器精确测量了对象的地形和速度,速度高达千米/秒. 这种先进的技术为详细分析提供可调节的时间灵敏度.

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Last Updated: Jun 12, 2025

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科学领域:

  • 光学和光子学 在光学和光子学.
  • 材料科学 材料科学 材料科学
  • 计量学 计量学是一门学科.

背景情况:

  • 量化相位成像对于非接触式计量学至关重要.
  • 高速物体表征需要先进的成像技术.
  • 数字全息显微镜 (DHM) 提供高分辨率的3D表面造型.

研究的目的:

  • 介绍一款新的数字全息显微镜诊断技术.
  • 为了能够同时量化地形和速度.
  • 为了实现动态测量可调节的时间灵敏度.

主要方法:

  • 从一个目标反射的双脉冲空间复杂化.
  • 使用具有精确的时间分离的参考光束.
  • 实施数字全息显微镜原理用于数据采集和处理.

主要成果:

  • 证明了对高速物体的地形学的量化.
  • 实现了物体以公里/秒的速度移动的速度测量.
  • 经过验证的可调时间灵敏度用于动态分析.

结论:

  • 开发的DHM诊断器能够进行高精度,高速的计量.
  • 这种技术促进了动态事件和快速移动的目标的表征.
  • 为需要详细对象分析的科学和工业应用提供了一种多功能工具.