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阿加深度对通过光盘扩散测试对抗微生物敏感性的影响

Ioana D Olaru¹, Sarah Schoeler¹, Frieder Schaumburg¹

¹Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.

Journal of medical microbiology

|September 18, 2024

概括

在抗微生物敏感性测试 (AST) 中,亚格尔深度的变化可能会影响结果. 虽然质量控制菌株可能保持在可接受的范围内,但临床隔离物显示出显著的错误,特别是非常大的错误,在非标准的深度.

科学领域:

临床微生物学临床微生物学
抗微生物耐药性抗微生物耐药性
诊断的准确性诊断的准确性

背景情况:

欧洲抗菌感受性测试委员会 (EUCAST) 建议在抗菌感受性测试 (AST) 中使用4±0.5毫米的标准化深.
在某些环境中,有限的资源可能会阻碍标准化粉的生产,这引发了人们对粉深度变化对AST准确度的影响的担忧.

研究的目的:

评估不同深 (2-6毫米) 对质量控制 (QC) 菌株抑制区直径和临床分离物AST准确性的影响.
评估不同亚格深处的区域直径与常见细菌病原体的最小抑制度 (MIC) 之间的关系.

主要方法:

测试的抑制区直径使用Mueller-Hinton II agar在深度范围为2毫米至6毫米的标准QC菌株 (大肠杆菌,P. aeruginosa,S. aureus) 和各种抗菌剂.
研究了来自塞拉利昂的35个临床分离物体的区域直径和MIC之间的相关性,包括大肠杆菌,肺炎,黄金和P. aeruginosa.

主要成果:

大多数QC菌株-抗菌组合在经过测试的亚格深度中产生了在EUCAST接受范围内的抑制区直径.
极端的亚格深度 (外部3.5-4.5毫米) 导致范围外抑制区的频率更高.
对临床分离物的抗微生物敏感性测试的准确性因亚格深度而异,显示了从85.8%到94.6%的绝对一致,从0.4%到2.1%的重大错误率和从3.3%到12.5%的非常重大错误率 (VME).

结论:

虽然QC菌株似乎可以产生可接受的结果,但深度的变化可能导致临床隔离试验中不可接受的高非常重大错误率 (>3%).
标准化的深对于可靠的AST至关重要,特别是在资源有限的环境中,以确保准确的抗微生物敏感性结果并指导有效的治疗.

关键词:

在 AST AST AST 中.对抗微生物的敏感性测试.磁盘扩散是指光盘的扩散.

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