一个新的小型滚筒电路,用于模拟体外循环
Anders K Hjärpe1,2, Anders Jeppsson3,4, Lukas Lannemyr2,5
1Department of Perfusion, Sahlgrenska University Hospital, Gothenburg, Sweden.
Interdisciplinary cardiovascular and thoracic surgery
|September 18, 2024
概括
一个新的微型电路有效地模拟了使用最少血液的体外循环. 与晶体溶液相比,基于德克斯的原始化显著损害了凝血,在模拟循环期间影响了血液静止.
科学领域:
- 心血管研究的心血管研究.
- 生物医学工程 生物医学工程
- 血液学 血液学 血液学
背景情况:
- 身体外循环 (ECC) 显著影响血液静血和风湿学.
- 活体模拟模型对于研究这些影响是有价值的,但往往需要大量的血液.
- 开发一个微型模型对于需要最小血液样本的研究至关重要.
研究的目的:
- 开发一个小型的滚筒电路,用于使用仅27毫升血液来模拟ECC的ex vivo模拟.
- 评估该电路在基于德克斯和基于晶体的原始化溶液之间比较凝血和血小板功能时的实用性.
主要方法:
- 创建了一个全新的微型滚筒电路.
- 来自8名心脏手术患者的血液样本在60分钟内循环使用基于德克斯或基于晶体的原始化溶液.
- 凝血 (旋转性血小板测量) 和血小板功能 (阻抗聚合计,流动细胞计) 在基线,5分钟和60分钟被评估.
主要成果:
- 两种原始化溶液都诱导了时间依赖的凝血损伤.
- 在5分钟和60分钟的时间内,基于德克斯的原始化导致与基于晶体的原始化相比,凝块度 (HEPTEM,EXTEM,FIBTEM) 显著降低.
- 基于德克斯的原始化导致5分钟和60分钟的EXTEM凝固时间显著延长.
结论:
- 开发的微型滚筒电路是ex vivo ECC研究的可行工具,血液需求最小.
- 在这个模型中,基于德克斯的原始溶液会导致血静的显著损害,而不是标准的晶体溶液.
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