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相关概念视频

Reproductive Cloning01:27

Reproductive Cloning

Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Reproductive Cloning01:27

Reproductive Cloning

Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Cloning of Dolly the Sheep01:08

Cloning of Dolly the Sheep

The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual,  the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter telomeres than other...
Design Example: Capacitance Multiplier Circuit01:20

Design Example: Capacitance Multiplier Circuit

In integrated circuit technology, a capacitance multiplier is often utilized to produce a larger capacitance value when a small physical capacitance falls short. This is achieved by a circuit that multiplies capacitance values by a factor of up to 1000, such that a 10-pF capacitor can replicate the performance of a 100-nF capacitor.
The circuit illustrated in Figure 1 below incorporates two op-amps, with the first operating as a voltage follower and the second acting as an inverting amplifier.
Clamper Circuit01:14

Clamper Circuit

A clamper circuit, also known as a DC restorer, represents a specialized variant of the rectifier circuit, notable for its method of taking the output across the diode rather than the capacitor. This configuration lends to several distinctive applications, particularly in handling square wave inputs.
Within this circuit, the diode's orientation prompts the capacitor to charge up to the level of the most negative peak of the input signal. Upon reaching this state, the diode ceases to conduct,...

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相关实验视频

Updated: May 10, 2026

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
08:25

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多绿:用于克隆GreenGate的多重复合架构.

Vincent J Pennetti1, Peter R LaFayette2, Wayne Allen Parrott1,2,3

  • 1Institute of Plant Breeding, Genetics and Genomics, University of Georgia, University of Georgia, Athens, Georgia, United States of America.

PloS one
|September 18, 2024
PubMed
概括

MultiGreen扩展了植物基因工程的GreenGate系统,使多个转录单元的高效组装成为可能. 这种新的克隆策略加速了复杂的植物转化载体的设计和原型设计.

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相关实验视频

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科学领域:

  • 植物生物技术 植物生物技术
  • 分子生物学分子生物学
  • 合成生物学 合成生物学

背景情况:

  • 像GreenGate这样的模块化克隆系统简化了植物向量设计.
  • 绿门的局限性是它无法有效地组装多个转录单元.

研究的目的:

  • 推出MultiGreen,这是GreenGate系统的扩展.
  • 为了克服GreenGate在串行和并行组装多个转录单元方面的局限性.

主要方法:

  • 开发了MultiGreen与1级接受器向量用于中间组装.
  • 使用数学模型来计算组装时间:2*log6n+3天.
  • 通过使用细菌操作子组合和RUBY报告员解构验证了MultiGreen.

主要成果:

  • 多绿色有效地连接多个转录单元.
  • 通过直接使用二进制MultiGreen1级向量实现了快速原型设计.
  • 证明了成功组装具有多个转录单元的复杂等离子体.

结论:

  • 多绿是一种多功能工具,用于复杂的克隆项目在植物转化.
  • 它保持了GreenGate的语法,同时启用了复杂化.
  • 加快多转录单元植物载体的设计和验证.