肺炎诊断中的元基因组学:系统审查议定书
Samuel Quarton1,2, Alana Livesey3, Charlotte Jeff1
1National Institute for Health Research Birmingham Biomedical Research Centre, Birmingham, United Kingdom.
JMIR research protocols
|September 18, 2024
概括
本系统性审查评估了用于肺炎诊断的下一代元基因组测序 (mNGS). 它将mNGS与社区获得的,医院获得的和呼吸机相关的肺炎类型的传统方法进行比较,以澄清临床益处.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 临床诊断 临床诊断 临床诊断
背景情况:
- 肺炎病原体的鉴定往往是有限的,影响了抗菌药物管理.
- 甲基因组下一代测序 (mNGS) 为病原体检测提供了更高的灵敏度.
- 在肺炎中mNGS的临床实用性,特别是在不同类型的肺炎和样本类型中,需要进一步定义.
研究的目的:
- 系统地审查和比较mNGS与传统微生物学方法的临床使用,以诊断社区获得的肺炎 (CAP),医院获得的肺炎 (HAP) 和呼吸机相关的肺炎 (VAP).
主要方法:
- 对Embase,MEDLINE,Scopus和Cochrane CENTRAL数据库进行系统的文献搜索,以比较成人肺炎患者的mNGS与传统微生物学的研究.
- 数据提取的重点是病原体阳性,周转时间,临床决策影响,停留时间和30天死亡率.
- 计划对肺炎类型 (CAP,HAP,VAP) 和样本类型进行子组分析;使用QUADAS-2评估偏差风险;对结果数据进行元分析或叙事综合.
主要成果:
- 最初的数据库搜索显示了5750个记录.
- 选和数据提取正在进行中,预计将于2024年9月完成.
结论:
- mNGS对肺炎患者护理途径的临床影响尚不清楚.
- 需要进一步的研究,以确定mNGS在不同类型的肺炎和样本类型中的好处,以便在常规临床中采用.
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