空腔H+-ATPase亚单元C参与了橄甲氨酸 (OG) 的内部化和OG触发的免疫
Moira Giovannoni1, Valentina Scafati1, Renato Alberto Rodrigues Pousada1
1Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100, L'Aquila, Italy.
Plant physiology and biochemistry : PPB
|September 18, 2024
概括
植物的免疫反应依赖于细胞壁碎片. 而DE-ETIOLATED 3 (DET3) 蛋白质对于识别这些碎片,启动防御信号和维持植物免疫力至关重要.
科学领域:
- 植物免疫力 植物免疫力
- 植物细胞壁的信号传递
- 分子植物病理学 分子植物病理学
背景情况:
- 植物的免疫反应是由损伤相关的分子模式 (DAMPs) 触发的,例如称为Oligogalacturonides (OGs) 的pectin碎片.
- 早期的研究在OG感知时发现了显著的蛋白质酸化变化,强调了研究特定蛋白质作用的必要性.
研究的目的:
- 阐明DE-ETIOLATED 3 (DET3) 的作用,它是真空H+-ATPase的一个子单元,它在由Oligogalacturonide (OG) 触发的植物免疫反应中的作用.
- 调查DET3对植物防御原料和病原体耐药性的影响.
主要方法:
- 使用了Arabidopsis det3敲击突变体来评估OG感知和下游信号.
- 分析了过氧化 (H2O2) 积累,基因激活蛋白激酶 (MAPK) 激活和防御基因表达,以应对OG.
- 评估了突变物对真菌病原体Botrytis cinerea的耐药性和OG预处理的影响.
- 采用光标记的OG来追踪它们在野生类型和DET3突变植物中的内细胞形成.
主要成果:
- 在OG治疗后,det3突变体显示H2O2积累,MAPK激活和防御基因诱导受损.
- det3突变体对Botrytis cinerea表现出增强的基底耐药性,后者通过OG预处理被取消.
- 与野生型植物相比,在det3突变体中,光标记的OG的内细胞分解效率明显低于野生型植物.
- 这些发现表明det3突变体的能力受到损害,维持了初始化的防御状态.
结论:
- 在植物中,DET3在橄甲氨酸 (OGs) 的内化和信号传递中起着至关重要的作用.
- 通过DET3调解适当的OG内部化对于启动和维持植物的原始免疫状态至关重要.
- 在DET3功能中的缺陷损害了植物防御初始化,影响了对DAMP和随后的病原体攻击的反应.
相关概念视频
Intralumenal Vesicles and Multivesicular Bodies
3.4K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.4K
Oligosaccharide Assembly
2.8K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
2.8K
Transcytosis of IgG
2.7K
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
2.7K
Overview of Secretory Vesicles
8.5K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.5K
Receptor Downregulation in MVBs
2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.0K
Clathrin Coated Vesicles
6.9K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
6.9K


