介质干细胞球体体的无标签活体表征通过生物物理性质测量
P Marrazzo1, A Sargenti2, R Costa3
1Department of Medical and Surgical Sciences, Alma Mater Studiorum University of Bologna, 40126, Bologna, Italy.
Advances in biological regulation
|September 18, 2024
概括
介酶体 stromal 干细胞球体 (MSph) 显示可变的质量密度和随着时间的推移下降的生存能力. 生物物理监测提供了一种方法来评估MSph的变异性,指导干细胞研究的最佳培养时间.
科学领域:
- 细胞生物学 细胞生物学
- 生物技术是生物技术.
- 干细胞研究 干细胞研究
背景情况:
- 三维 (3D) 细胞培养是干细胞研究中的一个关键方法,它能够形成介质干细胞干细胞球体 (MSph).
- MSph,也称为 mesenspheres,与介质层干细胞 (MSC) 有共同的特性,但根据细胞类型和组织来源表现出可变的形态和紧缩.
- 这种MSph生物物理性质的变化,例如质量密度,可以影响干细胞的功能,生存能力和实验协议中的可比性.
研究的目的:
- 研究MSph.中可变的生物物理性质所带来的局限性.
- 开发和验证一种方法来评估使用质量密度,重量和尺寸的MSph变异性.
- 为了评估天然化合物 (plumbagin) 对MSph生物物理性质的影响.
主要方法:
- 来自七个不同的组织来源的MSph的比较.
- 质量密度,重量和尺寸的综合评估与基于ATP的活力测试.
- 用plumbagin对选定的MSph进行治疗,以评估其对质量密度的影响.
主要成果:
- 在静态条件下培养的MSph随着时间的推移显示活力降低.
- 在不同细胞类型中,质量密度趋势在不同细胞类型之间有显著差异.
- 普伦巴治疗改变了特定MSph类型的质密度,证明了该方法在监测治疗后变化的有效性.
结论:
- 像质密度这样的生物物理性质对于理解MSph行为和变异性至关重要.
- 开发的生物物理方法有效地监测MSph变异性,包括对治疗的反应.
- 建议在形成后的早期使用MSph,以确保干细胞活力和表型保留.
更多相关视频
10:08Light-Induced Dielectrophoresis for Characterizing the Electrical Behavior of Human Mesenchymal Stem Cells
Published on: June 16, 2023
1.7K
08:43Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
Published on: August 9, 2024
862
相关概念视频
Characteristics of Life
233.0K
Biology is a natural science that studies life and living organisms, including their structure, function, development, interactions, evolution, distribution, and taxonomy. The field's scope is extensive and divided into several specialized disciplines, such as anatomy, physiology, ethology, genetics, and many more. All living things share a few key traits, including cellular organization, heritable genetic material and the ability to adapt/evolve, metabolism to regulate energy needs, the...
233.0K
Mechanical Protein Functions
4.4K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
4.4K
Studying the Cytoskeleton
8.3K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
8.3K
Cell Motility through Blebbing
1.9K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
1.9K
Protein Dynamics in Living Cells
1.9K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
1.9K
Methods to Assess Microbial Populations
100
Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a...
100
