在Citrus depressa Hayata液体发酵过程中代谢和微生物组的动态变化
Ta-Wei Liu1, Benni Iskandar1, Man-Hsiu Chu1
1School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, 11042, Taiwan.
Food chemistry
|September 18, 2024
概括
使用Bacillus amyloliquefaciens的液体发酵为Citri Reticulatae Pericarpium (CRP) 提供了一种稳定的加工方法,增强有益的聚甲基黄素 (PMF) 并提供对代谢物-微生物群相互作用的见解.
科学领域:
- 草药是一种草药.
- 微生物学 微生物学
- 代谢学 代谢学 代谢学
背景情况:
- (Citri Reticulatae Pericarpium,简称CRP) 是一种传统的中国草药,具有多样化的生物活性.
- 传统的加工方法容易受到时间,环境和微生物影响的变化,影响CRP质量.
- 了解代谢物-微生物群相互作用对于优化CRP生物转化至关重要.
研究的目的:
- 使用孤立的微生物开发一种更稳定的CRP液体发酵工艺.
- 研究在CRP加工过程中微生物群落和代谢物产生的相关性.
- 评估Bacillus amyloliquefaciens作为CRP的替代加工剂的潜力.
主要方法:
- 固态发酵被液体发酵所取代.
- 微生物群落使用长读序列分析.
- 使用非准代谢物分析分析了代谢物概况.
- 使用斯皮尔曼相关性分析,将代谢物与微生物联系起来.
主要成果:
- 液体发酵转移了占主导地位的细菌属,从Lactobacillus转变为Pediococcus,然后转变为Clostridium.
- 非向的代谢学发现了70种不同的代谢物,包括氨基酸,聚甲基黄素 (PMF) 和碳水化合物.
- 在特定的Clostridium物种和PMF生产之间观察到正相关性.
- 乙氨基菌发酵增加了PMF含量,但没有降低hesperidin水平.
结论:
- 用Bacillus amyloliquefaciens进行液体发酵,为CRP加工提供了一种稳定有效的方法.
- 这种方法增强了有益的PMFs的生产.
- 该研究提供了关于代谢组-微生物组相互作用的宝贵数据,用于未来草药中的生物转化研究.
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