沙门氏菌Typhimurium屏幕识别了肠道殖民期间混合酸发酵的转变
Bidong D Nguyen1, Anna Sintsova1, Christopher Schubert1
1Institute of Microbiology, Department of Biology, ETH Zürich, Zürich, Switzerland.
Cell host & microbe
|September 18, 2024
概括
沙门氏菌Typhimurium通过切换发酵途径,使其新陈代谢适应肠道环境. 这种代谢灵活性使病原体能够节省能量,并在感染期间殖民宿主.
科学领域:
- 微生物学 微生物学
- 代谢工程是代谢工程.
- 病原体与宿主之间的相互作用
背景情况:
- 了解肠道病原体如沙门氏菌 Typhimurium (S.Tm) 如何在动态的肠道环境中适应其新陈代谢对于开发有效的治疗方法至关重要.
- 肠道光膜呈现出不断变化的环境,营养物质的可用性和宿主免疫反应的波动.
研究的目的:
- 在肠道殖民期间使用体内方法研究沙门氏菌Typhimurium的代谢适应.
- 确定关键的代谢途径,使S.Tm能够在肠道中存活和生长.
主要方法:
- 在 gnotobiotic 鼠标模型中进行了 in vivo 转位子突变发生屏幕.
- 利用碳水化合物分析和测量葡萄糖生成来评估代谢状态.
- 分析了发酵产品和TCA循环活性.
主要成果:
- 混合酸发酵对于S.Tm的肠道光线生长和节能至关重要.
- 在最初的殖民化过程中,S.Tm采用了酸盐发酵和烟酸盐呼吸.
- 在炎症开始时,S.Tm转向乙醇发酵,并在六合酶变得有限时利用葡萄糖生成,为TCA循环提供α-甲酸作为能量.
结论:
- 沙门氏菌Typhimurium表现出代谢灵活性,适应其发酵策略和TCA循环使用,以在不断变化的肠道环境中壮成长.
- 这些适应性代谢能力对于STM成功的肠道殖民是必不可少的.
- 类似的代谢适应可能在其他致病性肠杆菌群中保持,这表明肠道殖民的共同机制.
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