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通过miR-665/FGF9通路,LINC00665会加剧软骨肉瘤细胞中的恶性表型

Jin Qian¹, Lujie Ge¹, Congcong Lu¹

¹Department of Orthopedics, Affiliated Hangzhou First People's Hospital, WestLake University School of Medicine, Hangzhou 310006, Zhejiang Province, China.

International journal of biological macromolecules

|September 18, 2024

概括

长跨基因非蛋白编码RNA 665 (LINC00665) 通过与microRNA-665 (miR-665) 和纤维细胞生长因子9 (FGF9) 相互作用,促进了软骨肉瘤的进展. 这一轴为软骨肉瘤治疗提供了潜在的新治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

长非编码RNAs (lncRNAs) 与瘤发生有关,但它们在冠状动脉瘤中的作用在很大程度上仍未被探索.
软骨肉瘤是一种罕见的骨癌,有效的治疗选择有限.

研究的目的:

调查长跨基因非蛋白质编码RNA 665 (LINC00665) 在软骨肉瘤的发展和进展中的作用.
阐明LINC00665在软骨肉瘤中的功能背后的分子机制.

主要方法:

定量实时PCR (RT-qPCR) 用于基因表达分析.
细胞增殖 (CCK-8,EDU),细胞亡 (TUNEL),迁移和入侵试验 (Transwell,伤口愈合).
RNA免疫沉 (RIP),光酶记者试验,以探索分子相互作用.

主要成果:

在冠状腺癌细胞系中,LINC00665的调节显著上升.
LINC00665干扰抑制了冠状腺癌细胞的增殖,迁移和入侵.
LINC00665充当了microRNA-665 (miR-665) 的海绵,导致纤维细胞生长因子9 (FGF9) 表达的增加,这推动了软骨肉瘤的进展.

结论:

LINC00665通过miR-665/FGF9轴促进了软骨肉瘤的进展.
向LINC00665/miR-665/FGF9通路可能代表着一种新的治疗策略.

关键词:

冠状腺肉瘤 (chondrosarcoma) 是一种癌症.FGF9 联邦政府财政基金 LINC0066565 这是一本书.核武器的扩散在 miR-66565 的情况下,

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