Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Genome-wide association studies of infant and toddler temperament in European and multi-ancestry populations.

Nature human behaviour·2026
Same author

Saturation Genome Editing reveals the functional impact of RAD51D <i>and</i> XRCC2 variants.

bioRxiv : the preprint server for biology·2026
Same author

A Retinoic Acid Autoregulatory Loop Governing Prefrontal-Motor Arealization.

bioRxiv : the preprint server for biology·2026
Same author

Gigabase-scale deletion scanning of the human genome.

bioRxiv : the preprint server for biology·2026
Same author

Phenotype-Specific Recalibration of MAVE Data Enables Repurposing of <i>BAP1</i> Functional Assays for Küry-Isidor Syndrome.

medRxiv : the preprint server for health sciences·2026
Same author

A comparative survey of functional evidence use in hearing and vision loss genetics.

Communications medicine·2026
Same journal

Kat5 deficiency in alveolar type II cells licenses STAT6-driven glycolytic reprogramming and pulmonary fibrosis.

Nature communications·2026
Same journal

Continuous nonthermal slab gap formed by progressive tearing beneath Northeast Asia.

Nature communications·2026
Same journal

Zeolitic isolated protonic acid sites-mediated NH<sub>3</sub> storage for robust NO<sub>x</sub> removal.

Nature communications·2026
Same journal

Coaxially nested component with asymmetric fiber resonant cavity and separation membrane for gaseous and dissolved gases detection.

Nature communications·2026
Same journal

Near-unity charge readout signal in a nonlinear resonator without matching the sensor dissipation.

Nature communications·2026
Same journal

Prokaryotic Schlafen proteins cleave tRNAs during type III CRISPR immunity.

Nature communications·2026
查看所有相关文章

相关实验视频

Updated: Jun 20, 2026

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
10:46

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

9.3K

多重,单细胞CRISPRa查细胞类型特定的监管元素.

Florence M Chardon1,2, Troy A McDiarmid1,2, Nicholas F Page3,4,5

  • 1Department of Genome Sciences, University of Washington, Seattle, WA, USA.

Nature communications
|September 18, 2024
PubMed
概括
此摘要是机器生成的。

这项研究介绍了一种基于CRISPR的基因激活 (CRISPRa) 框架,使用单细胞RNA测序来绘制细胞类型特定基因调节的地图. 该方法识别了可以精确控制特定细胞类型中的基因表达的调控元素,包括与神经发育障碍相关的基因表达.

更多相关视频

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.3K
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
00:09

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

21.8K

相关实验视频

Last Updated: Jun 20, 2026

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
10:46

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

9.3K
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.3K
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
00:09

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

21.8K

科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 基因规则 基因规则

背景情况:

  • 基于CRISPR的基因激活 (CRISPRa) 能够对基因表达进行有针对性的上调.
  • 识别细胞类型特定的调控元素对于理解基因控制至关重要.
  • 现有的方法缺乏分辨率,无法在不同类型的细胞中绘制精确的调节元件功能.

研究的目的:

  • 开发和验证一个实验框架来识别特定于细胞类型的CRISPRa-响应性cis-regulatory元素.
  • 发现能精确控制特定基因区域内邻近基因的表达的调节元件.
  • 用CRISPRa.来研究增强剂活性的细胞类型特异性.

主要方法:

  • 一个高度多重化的CRISPRa扰动系统与单细胞RNA测序 (sc-RNA-seq) 相结合.
  • 随机组合的指导RNA (gRNAs) 针对候选 cis-调节元素被引入细胞.
  • 细胞使用sc-RNA-seq进行了剖析,并分析了扰动,以评估测试对照组对基因表达的影响.

主要成果:

  • 该框架成功识别了gRNAs,这些gRNAs专门调高了预期的基因,而不会影响1Mb内的邻近基因.
  • 克里斯普拉α反应增强剂表现出细胞类型特异性的活性,表明细胞环境的依赖性.
  • 使用这种方法实现了神经元中六个自闭症谱系障碍 (ASD) 和神经发育障碍 (NDD) 风险基因的升级.

结论:

  • 开发的框架可以有效地发现细胞类型特定的调节元素及其向基因.
  • 增强剂的活性高度依赖于细胞类型,受染色体格局和转化作用因子的影响.
  • 这种方法促进了对CRISPR的大规模查,这些工具可以激活具有精确细胞类型特异性的基因.