相关实验视频

Updated: Jun 20, 2026

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
10:46

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

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多重,单细胞CRISPRa查细胞类型特定的监管元素

Florence M Chardon1,2, Troy A McDiarmid1,2, Nicholas F Page3,4,5

  • 1Department of Genome Sciences, University of Washington, Seattle, WA, USA.

Nature communications
|September 18, 2024
PubMed
概括

这项研究介绍了一种基于CRISPR的基因激活 (CRISPRa) 框架,使用单细胞RNA测序来绘制细胞类型特定基因调节的地图. 该方法识别了可以精确控制特定细胞类型中的基因表达的调控元素,包括与神经发育障碍相关的基因表达.

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