测试快速蒸发式质谱法用于组织组织分类和分子诊断在多地点研究中的测试
Martin Kaufmann1,2, Pierre-Maxence Vaysse3,4,5, Adele Savage6
1Department of Surgery, Queen's University, Kingston, ON, Canada.
British journal of cancer
|September 18, 2024
概括
快速蒸发电离质谱法 (REIMS) 可以在多个部位可靠地识别乳腺瘤. 这项技术在临床环境中对精确的瘤边缘评估具有前景.
科学领域:
- 生物医学工程 生物医学工程
- 质谱测量质量谱测量
- 在瘤学瘤学.
背景情况:
- 快速蒸发性电离质谱法 (REIMS) 已经显示出出活体乳腺瘤组织鉴定的潜力.
- 雷姆斯的稳定性和分子诊断能力需要进一步调查.
研究的目的:
- 评估在多个地点获取交叉可比的REIMS数据集的可行性,以识别乳腺瘤和亚型.
- 确定REIMS是否可以支持乳腺癌的分子诊断.
主要方法:
- 一个多站点的财团使用了相同的REIMS设置和协议进行组织分析.
- 分析了21个乳腺癌样本,使用统计方法比较正常,WT和PIK3CA突变管道癌.
主要成果:
- 虽然在标准分析中观察到站点间和用户间的变异性,但对于乳腺癌数据,多变量分类模型实现了高准确性 (97.1%98.6%).
- REIMS在不同部位的分子亚型,特别是PIK3CA突变方面表现出一致的结果.
结论:
- 基于REIMS的边际评估的全球分类模型是可行的,表明临床可翻译性.
- 雷姆斯技术显示承诺作为一个强大的工具,既用于组织学识别和乳腺瘤的分子亚型.
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