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来自联合短的美索和核特异性T细胞有效地杀死三阴性乳腺癌细胞

Suyanee Thongchot^1,2, Krittaya Aksonnam¹, Jaturawitt Prasopsiri¹

¹Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.

|September 18, 2024

概括

这项研究表明,将美索和核类结合起来,有效地产生T细胞以向三阴性乳腺癌 (TNBC). 这些工程T细胞表现出强大的杀癌能力,为TNBC提供了有前途的新疗法.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
细胞疗法细胞疗法

背景情况:

三阴性乳腺癌 (TNBC) 具有侵略性,治疗选择有限.
使用抗原特异性T细胞的采用细胞转移是一种有前途的策略.
梅索林 (MSLN) 和核林 (NCL) 在TNBC中过度表达,使它们成为潜在的治疗点.

研究的目的:

研究外围血液单核细胞 (PBMC) 的多脉冲的有效性,以产生MSLN/NCL特异性T细胞.
使用这些工程T细胞向MSLN+/NCL+TNBC细胞.

主要方法:

在TNBC患者样本中使用免疫组织化学证实MSLN和NCL表达.
来自健康捐赠者的活性PBMC与合成的MSLN和NCL.
通过晶体紫色染色,流动细胞计量和细胞因子珠阵列评估T细胞杀癌能力,亚型和细胞因子生产.

主要成果:

85.3%的TNBC病例表达MSLN或NCL或两者兼而有之.
多脉冲显著诱导了T_EM和T_EMRA CD3⁺/CD8⁺ T细胞和IFN-γ的产生.
在MSLN+/NCL+ TNBC细胞中,MSLN/NCL特异性T细胞诱导细胞死亡,并释放细胞毒性因子.

结论:

多脉冲PBMC是一种可行的方法,用于产生抗原特异性T细胞.
这种方法在TNBC中作为一种新治疗策略的概念验证.
准MSLN和NCL为TNBC治疗提供了潜在的新途径.

关键词:

免疫治疗是一种免疫疗法.梅索他林是其中一种蛋白质.核素核素是一种核素.短质是一种短质.三阴性乳腺癌是三阴性乳腺癌.

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