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在不同的胎盘疾病中,印制基因的甲基化改变
Xuwei Wang1,2, Yunyun Liu2, Yuying Wu3
1Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, No.63 Duobao Road, Liwan District, Guangzhou, 510150, Guangdong, China.
Clinical epigenetics
|September 18, 2024
概括
胎盘印记基因的表观遗传变化与先兆子 (PE) 和胎盘增生谱 (PAS) 疾病有关. 这些基因的异常DNA甲基化和表达模式表明PE和PAS在病变发生过程中发挥了不同的作用.
科学领域:
- 生殖生物学 生殖生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 印制的基因对胎盘发育和怀孕结果至关重要.
- 表观遗传修饰,特别是DNA甲基化,与胎盘疾病有关,如子前期 (PE) 和胎盘增生谱 (PAS) 障碍.
- 有限的研究存在于印记基因在各种胎盘病理中的特定作用.
研究的目的:
- 在PE和PAS中对DNA甲基化和胎盘印记基因的表达进行比较分析.
- 调查胎盘DNA氧化损伤与印记基因变异之间的关联.
- 评估胎儿生长标志物 (IGF2,皮质醇) 与PE和PAS中的胎盘基因概况相关.
主要方法:
- 使用双硫酸盐测序PCR对胎盘印记基因甲基化的比较分析.
- 定量PCR用于评估印制基因表达水平.
- 与酶相关的免疫吸收试验 (ELISA) 用于测量带血中的8-基-2'-脱氧氨酸,IGF2和皮质醇水平.
主要成果:
- 在PE和PAS胎盘中观察到KCNQ1,GNAS,MEST和IGF2的甲基化和表达的显著变化.
- 与正常怀孕相比,母亲的印记基因表达不同:在PE中表达过多,在PAS中表达不足.
- 增加的胎盘DNA氧化损伤与IGF2DNA甲基化有正相关;皮质醇和IGF2水平在PE和PAS中均下降.
结论:
- 异常的DNA甲基化和印记基因的表达是PE和PAS胎盘的特征.
- 在PE和PAS之间存在不同的DNA甲基化概况,这表明了独特的致病机制.
- 印记基因失调是PE和PAS不同的病理的潜在贡献者.
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