在接受NACT的乳腺癌患者中,血ccfDNA,mtDNA变化,CTC和上皮细胞-介质细胞过渡之间的相关性
Betül Çelik1, İrem Peker Eyüboğlu2, Sinan Koca3
1Department of Medical Biology and Genetics, Health Sciences Institute, Marmara University, İstanbul, Turkiye.
Turkish journal of medical sciences
|September 19, 2024
概括
无血细胞DNA (ccfDNA) 和线粒体DNA (mtDNA) 显示出作为乳腺癌生物标志物的前景. 水平随着治疗而变化,这表明监测化疗反应和疗效的潜力.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 乳腺癌需要非侵入性的血液生物标志物来选择治疗.
- 在乳腺癌患者中观察到血循环无细胞DNA (ccfDNA) 的升高.
- ccfDNA和线粒体DNA (mtDNA) 是血液中释放的碎片.
研究的目的:
- 调查ccfDNA和mtDNA作为乳腺癌治疗反应的指标.
- 探索ccfDNA和mtDNA作为监测治疗疗效的生物标志物.
- 将这些标记物与循环瘤细胞 (CTC) 数据进行比较,并评估与上皮-介质细胞过渡 (EMT) 的关系.
主要方法:
- 包括36名女性乳腺癌患者和21名健康女性.
- 使用定量聚合酶连锁反应 (qPCR) 来测量DNA水平 (ND1,ND4,ALU115,ALU247,GAPDH).
- 通过计算比率 (ALU247/ALU115和ND4/ND1) 来确定DNA完整性.
主要成果:
- 治疗后,患者的ccfDNA水平显著下降,mtDNA拷贝数 (mtDNAcn) 增加.
- 在治疗后没有观察到ccfDNA和mtDNA完整性的显著变化.
- 与对照组相比,患者的ALU115和ALU247水平较高,ccfDNA完整性较低.
结论:
- 这项研究全面研究了血ccfDNA,mtDNAcn和整体性,以及它们与CTC和治疗反应的关系.
- 结果表明血ccfDNA和mtDNA是评估化疗反应的潜在生物标志物.
- 这些标记物可以单独或组合使用,用于监测乳腺癌治疗疗效.
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