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机器学习和单细胞分析确定了与菌相关的基因TOMM22作为脊椎间盘退行症的潜在诊断生物标志物

Yinghao Wu¹, Shengting Wu¹, Zhiheng Chen¹

¹Department of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200233, PR China.

|September 19, 2024

概括

细胞过程中的线粒,在椎间盘变性中升高. 该TOMM22基因被确定为这种疾病的潜在诊断标记物.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
生物化学生化学

背景情况:

线粒细胞吸收有选择地去除受损的线粒体,防止细胞损伤和减少炎症.
线粒细胞衰变在椎间盘退化 (IDD) 中的作用以前没有被理解.

研究的目的:

为了调查 mitophagy 在 IDD 的参与.
评估IDD中菌相关基因的诊断价值.
为了确定IDD的潜在生物标志物.

主要方法:

利用GSVA,机器学习 (SVM-RFE,随机森林),CIBERSORT,MCPcounter,共识聚类和WGCNA来分析IDD中的菌体.
进行单细胞和伪时间分析,以检查细胞核细胞中的线粒分数和TOMM22表达.
在人类椎间盘组织中使用免疫组织化学和PCR验证了TOMM22表达.

主要成果:

与正常组织相比,在IDD中,线粒细胞衰变途径得分显著高于正常组织.
在线基因和免疫细胞透之间发现了强烈的相关性,这表明IDD类型的潜力.
与线粒细胞衰变相关的TOMM22基因在退化的圆盘细胞和组织中表达高,这表明它在IDD病变发生过程中的作用.

结论:

线粒在椎间盘退化中起着重要作用.
TOMM22是脊椎间盘退行症的有前途的诊断标志物,可能有助于疾病分类.

关键词:

人体组织样本验证验证椎间盘退化 (IDD) 是指椎间盘间的退化.机器学习是机器学习.线粒细胞衰变 (mitophagy) 是一种一个单细胞分析.

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