奎尔素通过稳定HIF1A蛋白质来缓解压缩诱导的细胞死亡和腰椎变性
Junxiao Ren1, Rui Xin1, Xiaoping Cui2
1The First Clinical Medical College of Yunnan University of Chinese Medicine, Kunming, 650500, Yunnan, China.
Heliyon
|September 19, 2024
概括
Quercetin 是传统中医药中的一种化合物,通过稳定 HIF1A,改善细胞存活率和减少疾病进展来保护腰椎退化.
科学领域:
- 综合生物学 综合生物学
- 传统中国医药 传统中国医药
- 再生医学是一种再生医学.
背景情况:
- 腰椎间盘退化 (LDD) 损害了细胞核脉动性介质干细胞 (NPMSCs).
- 沙甘 (Shaoyao-Gancao) (SGD) 是一种用于LDD的传统中医药,但其活性化合物和机制尚不清楚.
研究的目的:
- 通过网络药理学和转录组分析,识别针对LDD的SGD中的生物活性化合物.
- 为了研究素的治疗潜力,一个预测的活性成分,在LDD.
主要方法:
- 综合性网络药理学和转录组分析以确定针对LDD的SGD化合物.
- 在实验室中使用机械压缩对NPMSCs的研究.
- 在体内研究使用鼠标模型的椎间盘退化.
主要成果:
- 低氧诱导因子1-alpha (HIF1A) 被确定为昆塞丁在LDD中的关键标.
- 奎尔赛丁稳定了HIF1A,缓解了NPMSC中的氧化应激,细胞亡和细胞死亡.
- 在大鼠模型中,奎尔丁治疗改善了椎间盘退化.
结论:
- HIF1A是防止压缩诱导的NPMSC死亡的保护因素.
- Quercetin 通过 HIF1A 稳定,改善 NPMSC 的存活率,并减轻 LDD 的进展.
- 针对HIF1A通路使用诸如奎尔丁之类的天然化合物,为LDD提供了一个有前途的治疗策略.
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