评估对葡萄糖代谢对胰岛素抵抗HepG2细胞的影响
Changhao Li1, Ke Lin2, Liang Xiao1
1School of Public Health, Soochow University, Suzhou, 215123, China.
Heliyon
|September 19, 2024
概括
暴露改善了胰岛素抵抗性HepG2细胞的葡萄糖代谢,通过增强葡萄糖吸收和激活胰岛素信号. 然而,它也引起了氧化应激和线粒体损伤.
科学领域:
- 环境毒理学环境毒理学
- 代谢障碍 代谢障碍 代谢障碍
- 细胞生物学 细胞生物学
背景情况:
- (Cd) 是一种环境内分泌干扰物,与代谢功能障碍有关.
- 关于Cd对HepG2 (IR-HepG2) 细胞胰岛素抵抗的影响的数据有限.
- 由于污染,糖尿病患者中CD暴露很普遍.
研究的目的:
- 为了研究 (Cd) 对抗胰岛素的HepG2 (IR-HepG2) 细胞模型的代谢作用.
- 阐明Cd对葡萄糖代谢和细胞应激反应的影响的潜在机制.
主要方法:
- 使用棕酸建立了一个IR-HepG2细胞模型.
- 暴露于不同度 (1μM,2μM) 的化 (CdCl2) 的IR-HepG2细胞.
- 评估葡萄糖代谢,氧化应激 (ROS),线粒体膜潜力和胰岛素信号通路组件,通过商业套件,西部斑块和qRT-PCR.
主要成果:
- 暴露增强了IR-HepG2细胞的葡萄糖吸收并抑制了葡萄糖生成.
- 激活了胰岛素信号通路,并改善了IR-HepG2细胞中的葡萄糖代谢障碍.
- 诱导氧化应激,增加细胞内,抑制线粒体膜潜力,并导致补偿性糖解.
结论:
- 可以改善胰岛素抵抗性HepG2细胞中的葡萄糖代谢障碍.
- 会加剧线粒体损伤,并在IR-HepG2细胞中触发补偿性糖解.
- 这些发现为在胰岛素耐药性及其代谢后果中的作用提供了新的见解.
相关概念视频
Hormones Regulating Blood Glucose
3.1K
Insulin is released by beta cells of the pancreas when blood glucose levels are high. It facilitates glucose absorption and utilization in insulin-dependent cells with insulin receptors on their plasma membranes. Insulin promotes glucose uptake by increasing the number of glucose transport proteins in the cell membrane, allowing glucose to enter the cell. As a result, glucose utilization and ATP production are enhanced.
In addition to accelerating glucose uptake and utilization, insulin has...
In addition to accelerating glucose uptake and utilization, insulin has...
3.1K
Diabetes Mellitus: Type 2 and Gestational
2.2K
Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...
2.2K
Overview of Carbohydrate Metabolism
792
Carbohydrate metabolism is a fundamental biochemical process that ensures a constant supply of energy to living cells. The most important carbohydrate is glucose, which can be broken down via glycolysis to enter into the Krebs cycle and eventually lead to the production of ATP through oxidative phosphorylation.
Glucose transport into cells is facilitated by a family of transport proteins called GLUT (Glucose Transporters). GLUT4 is the primary glucose transporter for insulin-stimulated glucose...
Glucose transport into cells is facilitated by a family of transport proteins called GLUT (Glucose Transporters). GLUT4 is the primary glucose transporter for insulin-stimulated glucose...
792
Oral Hypoglycemic Agents: Biguanides and Glitazones
182
Biguanides, particularly metformin (Glucophage), are insulin sensitizers that enhance glucose uptake, thereby reducing insulin resistance. Unlike sulfonylureas, metformin doesn't prompt insulin secretion, which helps to curb hypoglycemia risk. Metformin is beneficial in treating conditions like polycystic ovary syndrome due to its insulin-resistance reduction capability. The drug's primary action involves curtailing hepatic gluconeogenesis, a significant contributor to high blood...
182


