使用表面增强的拉曼光谱学与事件波长分析进行快速和无标签的A型流感病毒亚型鉴定
RyeAnne Ricker1,2, Nestor Perea Lopez3, Mauricio Terrones3
1National Institute of Allergy and Infectious Diseases, National Institutes of Health, 50 South Drive, Building 50, Bethesda, MD 20892, USA.
Biomedical optics express
|September 19, 2024
概括
快速识别流感A病毒亚型对于预防流行病至关重要. 使用785nm激发波长与表面增强的拉曼光谱和机器学习显著改善了密切相关的流感A亚型H1N1和H3N2的分化.
科学领域:
- 生物物理学的生物物理.
- 病毒学 病毒学
- 频谱学是一种光谱学.
背景情况:
- 早期病毒鉴定对于患者治疗和流行病学监测至关重要.
- 快速识别A型流感病毒的亚型,特别是与禽流感相关的亚型,对于预防大流行至关重要.
- 表面增强的拉曼光谱 (SERS) 在与机器学习相结合时,可以提供无标签的,快速的病毒检测和识别.
研究的目的:
- 通过使用SERS来确定最优的激发波长,以区分高度相似的流感A病毒亚型.
- 为了比较532nm和785nm激发波长在分辨流感A亚型H1N1和H3N2的有效性.
主要方法:
- 利用表面增强的拉曼光谱法 (SERS) 来无标签检测流感A病毒亚型.
- 使用机器学习算法来分析光谱数据.
- 使用532nm和785nm激发波长比较光谱不相似性和识别准确性.
主要成果:
- 流感A亚型H1N1和H3N2的光谱显示,使用785nm激发与532nm相比,使用785nm激发的差异更大.
- 亚型识别曲线下的交叉验证面积 (AUC) 在785nm (0.95) 时高于在532nm (0.86) 时.
结论:
- 激发波长为785纳米的激发波长对于使用SERS和机器学习来区分密切相关的流感A病毒亚型是优越的.
- 这种方法对快速识别其他密切相关的病原体充满希望.
相关概念视频
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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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01:20Raman Spectroscopy: Overview
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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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