细胞单层和三维多细胞聚合物的温林张力的比较
Luni Hu1, Rick I Cohen1, Margarida Barroso2
1Department of Biomedical Engineering, Rutgers University, Piscataway, NJ 08854, USA.
Biomedical optics express
|September 19, 2024
概括
分子张力传感器揭示了2D和3D培养之间的细胞张力差异. 文库林张力传感器 (VinTS) 在3D聚合物中显示了较低的张力,这表明细胞环境发生了变化.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 生物材料是一种生物材料.
背景情况:
- 了解细胞张力对于组织工程和疾病研究至关重要.
- 文库林是一种关键蛋白质,参与细胞粘附和机械传导.
- 开发准确的方法来测量3D环境中的分子张力是具有挑战性的.
研究的目的:
- 为了比较3D多细胞聚合体与2D单层体内的中国仓鼠卵巢 (CHO-K1) 细胞中的温林张力.
- 为了研究3D微环境对文库林机械性能的影响.
- 在复杂的3D细胞培养中验证使用温库林张力传感器 (VinTS).
主要方法:
- 使用了对焦频域光寿命和福斯特共振能量转移 (FRET) 显微镜.
- 表达VinTS的细胞和一个没有尾巴的对照细胞 (VinTL) 在2D单层和3D多细胞聚合物中培养.
- 用Rho关联激酶抑制剂Y-27632治疗用于评估压力依赖FRET的效率.
主要成果:
- 与VinTL相比,VinTS在2D和3D培养中表现出较低的FRET效率,这表明紧张.
- 用Y-27632处理减轻了FRET效率差异,证实VinTS处于紧张状态.
- 与2D单层相比,在3D聚合物中观察到VinTS和VinTL的FRET效率显著下降.
- 帕克西林是一种已知的粘附蛋白,在2D中存在于VinTS部位,但在3D培养中基本上不存在.
结论:
- 在二维和三维细胞培养中都存在温古林张力.
- 与2D单层相比,3D多细胞聚合物环境改变了文库林的机械状态.
- VinTS是测量3D组织模型中的分子张力的一个可行的工具,尽管环境因素会影响其读数.
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