用Eurycoma longifolia提取物补充精子冷却介质可以提高泰国本土的精子质量和生育潜力
Thirawat Koedkanmark1, Ruthaiporn Ratchamak2,3, Supakorn Authaida1,2
1Department of Animal Science, Faculty of Agricultural, Khon Kaen University, Khon Kaen, Thailand.
Frontiers in veterinary science
|September 19, 2024
概括
将Eurycoma longifolia (EL) 提取物添加到精液扩展器中,可以通过减少氧化应激来改善冷藏期间的精液质量. 15毫克/毫升的度显示了增强精子生存能力和生育潜力的最有希望的结果.
科学领域:
- 生殖生物学 生殖生物学
- 生物化学 生物化学
- 动物科学动物科学
背景情况:
- 冷却的精液储存会诱导氧化应激,由于活性氧物种而对精液质量产生负面影响.
- 抗氧化剂对于缓解冷保存期间精子中氧化损伤至关重要.
研究的目的:
- 评估Eurycoma longifolia (EL) 提取物作为精液扩展器中抗氧化剂的疗效.
- 确定EL提取物在冷藏期间保持精液质量和生育潜力的最佳度.
主要方法:
- 在IGGKPh精液扩展剂中评估EL提取物的抗氧化能力,使用在不同度和温度下的2,2-二-1-皮克里利得拉齐尔试验.
- 用EL提取物 (5,10,15,20毫克/毫升) 补充精液扩展剂,并将精液在5°C下存储长达48小时.
- 评估的精液质量参数,包括麦隆迪甲水平和抗氧化酶活性.
- 在储存的精液上进行了生育和化能力测试.
主要成果:
- EL提取物表现出剂量依赖的抗氧化能力,在5°C化时得到增强.
- 补充15mg/ml的EL提取物显著降低了冷却精液中的马隆迪阿尔海德水平.
- 与对照组相比,在储存24小时的15 mg/mL EL提取物组中观察到更高的生育率.
- 在48小时后,抗氧化酶活性,化能力或生育能力没有显著差异.
结论:
- 尤里科马长叶草提取物,特别是15毫克/毫升,显示出在冷藏期间改善精液质量的潜力.
- 埃尔提取物可以在冷却的精液中提供抗氧化应激的保护作用,增强短期生育能力.
- 需要进行进一步的研究,以优化EL提取物的应用,以长期保存精液和提高生育能力.
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