基于生物信息学工具的组合分析揭示了与母亲肥胖和胎儿编程相关的新特征基因
Chunhong Liu1,2,3, Yulan Lu4, Chunchuan Huang1,2,3
1Center for Medical Laboratory Science, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
Frontiers in medicine
|September 19, 2024
概括
孕产妇的肥胖通过不清楚的分子路径影响胎儿的发育. 这项研究确定了与母亲肥胖和胎儿编程相关的胎盘组织中的五种新型特征基因 (PTX3,NCF2,HOXB5,ABCA6,C1orf162),其中HOXB5与出生体重有关.
科学领域:
- 基因组学和生物信息学
- 孕产妇和胎儿医学 孕产妇和胎儿医学
- 发展生物学 发展生物学
背景情况:
- 孕产妇肥胖对胎儿发育和长期健康构成重大风险.
- 连接母亲肥胖与胎儿编程的潜在分子机制在很大程度上是未知的.
- 了解这些机制对于改善围产期结果和终身健康至关重要.
研究的目的:
- 识别与孕产妇肥胖和胎儿编程相关的特征基因.
- 通过对胎盘组织的全基因组转录分析来研究分子机制.
- 探索发现的基因与新生儿出生体重之间的相关性.
主要方法:
- 使用了基因表达综合 (GEO) 数据集 (GSE128381),比较正常的BMI和肥胖的母亲.
- 采用生物信息学工具,包括用于差异表达基因 (DEG) 的limma,用于基因共同表达网络分析的WGCNA和用于特征基因选择的LASSO.
- 进行了简单的线性回归和基因组丰富分析 (GSEA),以评估基因与出生体重和信号通路的关联.
主要成果:
- 在正常和肥胖母亲之间确定了167个DEG (122个上调,45个下调).
- WGCNA强调"MEbrown"模块对孕产妇肥胖和胎儿编程具有重要意义.
- 拉索分析确定PTX3,NCF2,HOXB5,ABCA6和C1orf162作为关键的特征基因,在肥胖母亲的胎盘中升高;HOXB5与新生儿出生体重有显著的关联.
结论:
- PTX3,NCF2,HOXB5,ABCA6和C1orf162是涉及母亲肥胖和胎儿编程的新型特征基因.
- 具体来说,HOXB5的表达与新生儿出生体重的变化有关.
- 这些发现为对母亲肥胖对胎儿发育的影响提供了新的分子洞察力.
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