在植物中,FYVE1/FREE1参与了对谷氨酸敏感的TORC1激活
Mirai Tanigawa1,2, Tatsuya Maeda1, Erika Isono2,3
1Departments of Biology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka 431-3125, Japan.
iScience
|September 19, 2024
概括
科学家们确定FYVE1/FREE1是植物对关键氨基酸谷氨胺的传感器. 这种蛋白质直接激活了拉巴胺复合体1 (TORC1) 的标,调节细胞代谢和生长.
科学领域:
- 植物生物学 植物生物学
- 分子和细胞生物学分子和细胞生物学
- 生物化学 生物化学
背景情况:
- 拉巴胺素复合体1 (TORC1) 的标是细胞代谢的关键调节者,集成营养可用性等环境信号.
- 氨基酸在哺乳动物和植物中激活TORC1,但由于缺乏保存的Rag复合体,植物中的感知机制仍然不清楚.
- 了解植物氨基酸感应对于理解生长调节和营养信号至关重要.
研究的目的:
- 研究植物中氨基酸反应TORC1激活的分子机制.
- 为了确定涉及TORC1.1.上游检测氨基酸的潜在植物同类.
- 阐明阿拉比多普西斯FYVE1/FREE1在谷氨酸诱导的TORC1信号传递中的作用.
主要方法:
- 酵母-两个混合测试检测蛋白质-蛋白质相互作用.
- 谷氨胺治疗和西部血栓检测,以评估TORC1活动.
- 对过度表达野生类型和突变FYVE1/FREE1.1的转基因阿拉比多普西斯系的分析.
- 阿拉比多普西斯FYVE1/FREE1和酵母Pib2之间的域结构比较.
主要成果:
- 阿拉比多普西斯FYVE1/FREE1在对谷氨酸的反应中与TORC1相互作用.
- FYVE1/FREE1功能独立于其以前已知的与ESCRT相关的角色.
- 缺乏假定TORC1激活动机的FYVE1/FREE1变体的过度表达损害了谷氨酸诱导的TORC1激活.
- FYVE1/FREE1与酵母谷氨胺传感器Pib2.2共享域名相似之处.
结论:
- 阿拉比多普西斯FYVE1/FREE1作为氨基酸谷氨的细胞内传感器.
- 在植物中,FYVE1/FREE1直接触发TORC1激活,以应对谷氨胺.
- 这一发现揭示了植物中TORC1上游氨基酸感应的新机制,与Rag复杂途径不同.
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