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在稀有样本中发现低丰度细胞表面蛋白质的有效策略

Xiaoqiu Wu¹, Yuqing Liu^1,2,3, Dengwei Zhang^1,2,3

¹Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China.

Journal of the American Chemical Society

|September 19, 2024

概括

我们开发了一种新型的aptamer选择方法 (DiDS选择),用于识别低丰度细胞表面蛋白的分子识别探针. 这种技术使得精确的诊断和细胞分析成为可能,即使样本有限.

科学领域:

生物技术
分子生物学
诊断方法

背景情况:

在诊断和治疗中,aptamers是细胞表面蛋白质的关键分子识别探针.
在现场对低丰度蛋白质进行选择,特别是从稀缺的样本中选择,是一个重大挑战.

研究的目的:

提出一种单一的单细胞体选择方法 (DiDS选择),以克服体发现的局限性.
通过广泛的表达水平来识别向细胞表面蛋白的aptamer.

主要方法:

开发了数字DNA测序 (DiDS) 选择,一种单一循环,单细胞体选择策略.
纳入每个DNA模板的分子识别卡以减轻PCR放大偏差.
应用于细胞系,临床样本和神经元的DiDS选择.

主要成果:

通过使用DiDS选择成功生成了针对各种细胞类型和临床样本的高质量体.
与多轮选择偏好高丰度标不同,DiDS选择的标识了多种丰度标的蛋白质.
单细胞水平的细胞表面造型揭示了每个细胞系的独特分子模式,使用丰富的体池.

结论:

DiDS选择是一种简化方法,用于快速生成针对细胞表面蛋白的特定识别分子.
这种方法对细胞分析,特定探针识别和生物标志物发现具有前景.
能够对低丰富度的体进行选择,从而扩大精准医学中的应用.

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