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具有核样转移酶或非正规棕,催化域的蛋白质的结构和进化分析

Rodrigo Jácome¹

¹Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, México. rodrigo.jacome@ciencias.unam.mx.

Journal of molecular evolution

|September 19, 2024

概括

三级结构分析揭示了整个生命中蛋白质折叠的保存,即使初级序列分离. 这种结构性保护凸显了核样转移酶折叠的古老起源和功能多样性.

科学领域:

生物化学生物化学
结构生物学结构生物学
进化生物学进化生物学

背景情况:

许多必不可少的酶利用核基转移酶 (NT) 折叠,其特点是保存的酸性残留物协调金属离子.
蛋白质主要结构的进化分析可能是具有挑战性的,因为在长的进化时间尺度上有序列分歧.

研究的目的:

研究具有NT折的蛋白质的进化史和功能多样化.
为了证明基于三级结构的分析对研究蛋白质进化的实用性,当初级序列数据有限时.

主要方法:

使用PDBefold网络服务器对蛋白质与NT折叠进行对对结构比较,计算RMSD,叠加残留和Q-score.
导出结构对齐得分和1-Q得分来构建距离矩阵.
从距离矩阵生成树状图和系谱网络,以可视化进化关系.

主要成果:

基于三级结构的族系学分析揭示了具有高度结构保护的明确分类,在初级序列中并不明显.
确定了一个被保留的结构核心 (NT折叠),周围环绕着可变的功能域,导致不同的基质结合和功能.
证明具有NT折叠的蛋白质存在于生命的所有领域,表明它们的古老起源和基本作用.

结论:

基于三级结构的进化分析是发现蛋白质家族的进化关系和功能多样化的强大工具,特别是当初级序列显著分离时.
NT折叠是一种古老且高度保存的结构图案,对众多细胞和病毒功能至关重要.
结构性保护提供了超越初级序列信息的蛋白质功能的演变的见解.

关键词:

深刻的进化事件发生在进化过程中.核样转移酶折叠的核样转移酶折叠聚合酶是一种聚合酶.结构演变的结构演变.基于结构的分类学.

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