新型贝卡特病毒vB-BcgM的特征及其在食品矩阵中的抗菌作用
Yunfei Gong1, Lin Sun1, Xiaofu Wan1
1College of Life Sciences, South-Central Minzu University, Wuhan, 430074, China.
Archives of virology
|September 19, 2024
概括
一种新的菌体,vB-BcgM,有效地感染和复制Bacillus cereus组细菌中的细菌. 这种菌体在各种食品中显示出作为天然抗菌剂的巨大潜力.
科学领域:
- 微生物学 微生物学
- 病毒学 病毒学
- 食品科学 食品科学 食品科学
背景情况:
- 细菌Bacillus cereus群的细菌是导致食物腐烂和食物传播疾病的常见原因.
- 菌体是感染细菌的病毒,正在作为抗菌剂进行探索.
研究的目的:
- 为了隔离和描述一种针对Bacillus cereus群的新型细菌菌体.
- 评估这种菌体作为食品安全剂的潜力.
主要方法:
- 分离和细菌体vB-BcgM.的基因组特征.
- 在B. cereus群隔离物中确定宿主范围.
- 评估感染的效率,复制和稳定性.
- 在多种食物矩阵中评估抗菌功效.
主要成果:
- 一种贝卡特病毒的vB-BcgM感染了33.3%的B. cereus组菌株,包括B. cereus,B. thuringiensis和B. anthracis.
- 菌体表现出快速吸附,高复制能力 (爆裂大小为63 PFU/细胞),在广泛的pH (4-12) 和温度 (高达70°C) 范围内保持稳定.
- 在各种加工食品和新鲜食品中观察到显著的抗菌作用,这取决于剂量,食物矩阵,温度和时间.
结论:
- 菌体vB-BcgM是控制食品中B. cereus群污染的有希望的候选者.
- 其广泛的宿主范围,高效的感染和稳定性使其成为食品应用中可行的天然抗菌剂.
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