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纽卡斯尔病活病毒疫苗在各种储存条件下安全有效
Iffat Huma1, Shahan Azeem2, Sarwat Naz3
1Veterinary Research Institute, Lahore, Pakistan; Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Veterinaria italiana
|September 19, 2024
概括
这项研究评估了活衰减的纽卡斯尔病病毒 (NDV) 疫苗的热稳定性和纯度. 研究结果表明,该疫苗是纯粹的,有力的,有效的,尽管建议保持冷链.
科学领域:
- 兽医病毒学 兽医病毒学
- 免疫学 免疫学 免疫学
- 分子诊断学 分子诊断
背景情况:
- 纽卡斯尔病 (ND) 对家禽健康和全球粮食安全构成重大威胁.
- 活衰减疫苗对于控制ND至关重要,但它们的稳定性和纯度至关重要.
- 热稳定性和NDV疫苗的遗传特征确保了一致的保护.
研究的目的:
- 为了评估活衰减的NDV Mukteswar菌株疫苗的热稳定性.
- 对疫苗种子病毒进行基因特征,并评估其与现场分离的基因关系.
- 通过检测外来剂来确定疫苗的纯度.
主要方法:
- 在不同温度 (4°C,25°C,37°C) 和时间点进行热稳定性测试.
- 用血凝抑制 (HI) 试验对 brojler 进行功效和疗效评估.
- 通过针对F基因的RT-PCR进行基因表征和植物遗传学分析.
- 使用优化PCR面板对外来物质进行纯度测试.
主要成果:
- 该疫苗在经过测试的条件下储存后,证明了热稳定性,并保持了强度和有效性.
- 遗传学分析证实了疫苗病毒 (GenBank Acc. 没有,没有. MK310260,MK310261) 与NDVII类基因型III相关,但与巴基斯坦基因型XIII不同.
- 通过PCR检测证实,没有被测试的外来病原体 (禽类腺病毒,菌体,沙门氏菌).
结论:
- 活体减弱的NDV Mukteswar菌株疫苗是纯粹的,强效的和有效的.
- 可以使用基于PCR的快速方法来评估NDV活疫苗的纯度.
- 建议在疫苗储存期间保持冷链,以确保最佳的效能和有效性.
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