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Updated: Jun 12, 2025

Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation
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Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation

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如何冷电磁器彻底改变了生物能量学领域

Muratha Sottatipreedawong1, Ahad Ali Kazmi1, Irene Vercellino1

  • 1Ernst RuskaCentre 3 for Microscopy and Spectroscopy with Electrons, Forschungszentrum Jülich GmbH, Wilhelm-Johnen-Straße 52428 Jülich (DE).

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|September 19, 2024
PubMed
概括

电子显微镜 (cryo-EM) 彻底改变了结构生物学,使复杂的生物能量酶的详细研究成为可能. 这种技术为能量转化机制提供了关键的见解,特别是人类的氧化酸化系统.

关键词:
在OXPHOS复合体中.生物能源生物能源学低温电磁波冷却器 (Cryo-EM) 是一个非常好的方法.决议 革命 革命 革命

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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