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Updated: Jun 12, 2025

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
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发展微生物鉴定策略 - - 对生物化学,MALDI-TOF MS和分子测试方法的全面审查

Sophie S Arbefeville1, Tristan T Timbrook2,3, Cherilyn D Garner2

  • 1Microbiology & Molecular Pathology, Marshfield Clinic Health System, 1000 N. Oak Ave., Marshfield, WI 54449, USA.

The Journal of antimicrobial chemotherapy
|September 19, 2024
PubMed
概括

快速识别微生物对于及时做出临床决定和有效治疗至关重要. 本综述比较了传统和现代的病原体检测实验室方法,强调了它们在临床环境中的优缺点.

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Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment Kartchner Caverns, AZ, USA11:09

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相关概念视频

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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