Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA Editing02:23

RNA Editing

8.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
8.9K
Proofreading01:43

Proofreading

53.9K
Overview
53.9K
Base Excision Repair01:54

Base Excision Repair

22.2K
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
22.2K
DNA Base Pairing02:27

DNA Base Pairing

27.1K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
27.1K
Mismatch Repair01:20

Mismatch Repair

4.8K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
4.8K
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

7.0K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Specific SLC25 carriers regulate mitochondrial protein synthesis.

Science advances·2026
Same author

POLRMT overexpression increases mtDNA transcription without affecting steady-state mRNA levels.

Life science alliance·2025
Same author

Mitochondrial damage in muscle specific PolG mutant mice activates the integrated stress response and disrupts the mitochondrial folate cycle.

Nature communications·2025
Same author

The Vsr-like protein FASTKD4 regulates the stability and polyadenylation of the MT-ND3 mRNA.

Nucleic acids research·2024
Same author

Interorganelle phospholipid communication, a house not so divided.

Trends in endocrinology and metabolism: TEM·2024
Same author

Unique architectural features of mammalian mitochondrial protein synthesis.

Trends in cell biology·2024
Same journal

Rational Design of Linalool Dehydratase-Isomerase Enables Efficient Conversion of Phytol to Neophytadiene.

ACS synthetic biology·2026
Same journal

<i>De Novo</i> Biosynthesis of Polyphyllin V in <i>Nicotiana benthamiana</i> through Pathway Reconstruction and UDP-Sugar Engineering.

ACS synthetic biology·2026
Same journal

Rapid and Continuous Directed Evolution in <i>Vibrio natriegens</i> Utilizing an <i>In Vivo</i> Hypermutation System.

ACS synthetic biology·2026
Same journal

Machine Learning for Microbial Cell Factories: Pathway Design, Enzyme Engineering, and Metabolic Regulation.

ACS synthetic biology·2026
Same journal

Microfluidics-Based Engineering of Molecular Self-Assembly and Manufacturing for Artificial Cell Systems.

ACS synthetic biology·2026
Same journal

Beyond Compartmentalization: Deciphering Reaction Kinetics in Liquid-Liquid Phase Separation for Rational Biotechnological Design.

ACS synthetic biology·2026
查看所有相关文章

相关实验视频

Updated: Jun 12, 2025

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication
05:33

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Published on: July 5, 2024

594

过度活跃的尼克酶活动改善了腺基编辑.

Andrianto P Gandadireja1,2,3,4, Pascal D Vos1,2,3,4, Stefan J Siira4,5,6

  • 1Curtin Medical School, Curtin University, Bentley, Western Australia 6102, Australia.

ACS synthetic biology
|September 19, 2024
PubMed
概括
此摘要是机器生成的。

研究人员使用TurboCas9 nickase开发了一种改进的腺基编辑器 (ABE). 这种TurboABE提高了腺因 (A) 转化为关氨酸 (G) 的转化效率,而不增加非目标编辑,从而使更精确的基因编辑成为可能.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?这就是Cas9的情况.腺基编辑器 腺基编辑器基因编辑 基因编辑蛋白质工程工程 蛋白质工程

更多相关视频

A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

2.6K
RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
09:19

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Published on: July 22, 2014

8.4K

相关实验视频

Last Updated: Jun 12, 2025

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication
05:33

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Published on: July 5, 2024

594
A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

2.6K
RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
09:19

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Published on: July 22, 2014

8.4K

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物技术是生物技术.

背景情况:

  • 基编辑可以在没有双链断裂的情况下进行精确的DNA修改.
  • 腺基编辑器 (ABE) 将腺 (A) 转化为关氨酸 (G).
  • 目前的ABE面临由于除酶可用性和可变编辑效率的限制.

研究的目的:

  • 为了增强基因基因编辑器的基因组编辑活动.
  • 为了提高腺素 (A) 到关氨酸 (G) 转换的效率.
  • 克服现有的ABE在准特定DNA序列方面的局限性.

主要方法:

  • 将TurboCas9尼克酶纳入腺基编辑系统.
  • 在各种腺因位上对修改编辑器 (TurboABE) 的评估.
  • 评估编辑效率和对DNA和RNA的非目标效应.

主要成果:

  • 开发的TurboABE在多种不同的腺因点网站上显示了显著增强的编辑效率.
  • 在编辑窗口内,TurboABE在通常低效的站点改进了编辑频率.
  • 在DNA或RNA中没有观察到非目标编辑的增加.

结论:

  • TurboABE代表了基础编辑技术的进步,提供了更高的效率和精度.
  • 这种增强的ABE系统扩大了活细胞精确基因修饰的可能性.
  • 该开发解决了当前腺基编辑工具的局限性.