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Updated: Jun 12, 2025

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在Escherichia coli中,在适应重复的长期饥饿的过程中,pH敏感的转录终结的演变

Sarah B Worthan^1,2,3, Robert D P McCarthy¹, Mildred Delaleau⁴

¹Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232.

Proceedings of the National Academy of Sciences of the United States of America

|September 19, 2024

概括

在Rho转录终端的特定突变有助于细菌适应波动的环境. 这种Arg到His在Rho中的替代,加上YdcI的损失,使细胞能够快速协调对pH变化的反应.

科学领域:

微生物学微生物学
分子生物学分子生物学
进化生物学进化生物学

背景情况:

细胞群体在周期性压力波动的环境中面临挑战.
快速的细胞反应协调对于在不断变化的条件下壮成长至关重要.
在微生物群体的饥饿恢复周期中,环境pH值的波动发生.

研究的目的:

确定使细胞适应波动的环境的遗传因素.
研究Rho转录终端突变在环境适应中的作用.
了解Rho和YdcI在pH恒温和细胞可塑性中的相互作用.

主要方法:

在长期饥饿条件下,大肠杆菌的实验进化.
超基因组测序用于识别进化种群中的突变.
基因重建Rho R109H和DydcI突变,以评估它们的功能影响.

主要成果:

在Rho转录终结器 (rho R109H) 中发现了一种频繁的突变 (Arg到His替代).
携带rho R109H的群体也携带了 ydcI 的功能丧失突变, ydcI 是 pH 稳态调节剂.
罗R109H突变通过降低性条件下的Rho功能来赋予可塑性,与ΔydcI结合时导致化.

结论:

在Rho中Arg对His的替代作用为pH传感器,使生理反应的快速协调成为可能.
rho R109H 和 ΔydcI 组合导致 Rho 突变函数的遗传同化.
细胞群体利用环境线索,如pH值波动来协调适应性反应.

关键词:

性压力是一种性压力.实验进化的实验进化.允许的突变是允许的.现型性可塑性现型性可塑性转录终止转录终止

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