由Komagataella phaffii生产的发酵衍生牛乳酸的结构和功能
Emma C Skoog1, Vanessa Feher Castagna1, Shafraz Omer1
1TurtleTree Labs, PTE, LTD, Woodland, CA 95695, USA.
Biochemistry and cell biology = Biochimie et biologie cellulaire
|September 19, 2024
概括
通过精密发酵生产的重组牛乳酸 (rbLf) 在结构和功能上与原生牛乳酸 (bLf) 类似. 这种生物类型的rbLf为婴儿和成人健康产品提供了扩展的应用.
科学领域:
- 生物技术是生物技术.
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
背景情况:
- 牛乳酸 (bLf) 具有显著的健康益处,但受到自然丰度低和生产成本高的限制.
- 精密发酵为生产重组牛乳酸 (rbLf) 提供了一种可行的方法,提高了其用于各种应用的可用性.
研究的目的:
- 为了确认在*Komagataella phaffii*中产生的rbLf与本地bLf的结构和功能相似性,以符合监管要求和扩大市场.
- 验证rbLf作为一种与本地bLf生物类似的替代品,确保可比的疗效和安全性.
主要方法:
- 使用完整质量分析和LC-MS N-linked glycan分析来比较rbLf和bLf的分子量和糖化模式.
- 同电点和核心氨基酸序列分析证实了rbLf和bLf之间的相同性.
- 功能性测试评估了rbLf的铁结合能力,受体相互作用,上皮细胞生长促进,抗菌活性对抗大肠杆菌,脂多糖 (LPS) 结合,以及LPS诱导的类似收费受体4对抗性.
主要成果:
- rbLf的分子量 (84 kDa) 与bLf (82-83 kDa) 相似,具有相同的同电点和核心氨基酸序列.
- 糖甘分析显示,在rbLf上,主要是高曼诺斯基糖甘,类似于bLf糖甘的很大一部分.
- rbLf表现出保留的功能活动,包括铁结合,上皮细胞生长促进,大肠杆菌抑制,LPS结合和对抗LPS诱导的TLR4活性,具有统计学意义的结果 (P < 0.0001到P < 0.001).
结论:
- 结构和功能数据强烈支持rbLf和本地bLf之间的实质性相似性.
- 通过精密发酵生产的生物相似rbLf是一种可行的,高价值的成分,有可能在婴儿营养和成人健康产品中得到广泛使用.
- 这项研究证实rbLf是对本地bLf的有希望的替代品,为增加市场可访问性和应用铺平了道路.
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