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Updated: Jun 12, 2025

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Published on: October 19, 2018

葡萄卡拉芬和硫福拉芬减轻TNFα诱导的Caco-2单层透和炎症

Wei Zhu¹, Eleonora Cremonini¹, Angela Mastaloudis²

¹Department of Nutrition, University of California, Davis, CA, USA.

|September 19, 2024

概括

葡萄糖 (GR) 和硫 (SF) 通过减少炎症和透性来保护肠道屏障. 这些在西兰花中发现的化合物调节氧化还原平衡和信号通路,为慢性肠道炎症提供健康益处.

科学领域:

胃肠道学和营养学
分子生物学和生物化学分子生物学和生物化学

背景情况:

肠道透是慢性肠道炎症的一个关键因素.
十字花蔬菜含有葡萄糖 (GR) 和硫黄 (SF),这些化合物具有潜在的抗炎性质.

研究的目的:

研究GR和SF在缓解Caco-2细胞单层中瘤亡因子α (TNFα) 诱导的炎症和透性的疗效.
探索氧还原敏感事件和特定信号通路在GR和SF的保护作用中的作用.

主要方法:

用TNFα,GR和SF对Caco-2细胞单层的处理.
测量氧化剂的产生,NOX1,NOX4和Duox2.2的mRNA水平.
对NF-κB和MAPK ERK1/2信号通路的分析.
评估紧结 (TJ) 蛋白质水平 (occludin,ZO-1) 和肌轻链激酶mRNA.
分子对接模拟用于预测GR-TNFα受体相互作用.

主要成果:

剂量依赖的GR和SF减轻了TNFα诱导的氧化剂产生和NOX基因表达的增加.
这两种化合物都抑制了NF-κB和MAPK ERK1/2信号通路的激活.
GR和SF阻止了TNFα诱导的Caco-2单层透,并保持了TJ蛋白水平.
分子对接表明GR可以与NOX1相互作用,可能抑制其活性.

结论:

通过调节对氧化还原敏感通路,GR和SF有效地保护肠道屏障免受TNFα诱导的炎症和透.
GR,不仅仅是其衍生品SF,在保护肠道屏障完整性方面表现出生物活性.
饮食中摄入富含GR/SF的食物可能有助于减轻慢性肠道炎症及其后果.

关键词:

障碍物透性的障碍物葡萄糖甲氨酸是什么葡萄糖甲氨酸是什么肠道屏障肠道屏障肠道屏障肠道炎症是一种肠道炎症.硫甲是一种硫甲.瘤亡因子α因子

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