在GABAergic内神经元中状态调节的转录组对应物:跨物种分析
Joram Keijser1,2, Loreen Hertäg3, Henning Sprekeler3,4
1Modelling of Cognitive Processes, Technical University of Berlin, 10587 Berlin, Germany keijser@tu-berlin.de joramkeijser@gmail.com.
概括
研究人员探索了大脑细胞基因表达如何与物种间的行为状态有关. 他们发现物种之间的基因表达模式存在显著差异,影响大脑电路的功能.
科学领域:
- 神经科学是一个神经科学.
- 基因组学就是基因组学.
- 比较生物学的比较生物学
背景情况:
- GABAergic抑制性内部神经元表现出不同的亚型,具有不同的特性.
- 在小鼠视觉皮层中,内部神经元状态调制与基因表达相关,由第一个主要组成部分 (PC) 可预测.
研究的目的:
- 为了研究转录组与内神经元中状态依赖处理之间的联系是否在物种之间得到保护.
- 在哺乳动物,鸟类和爬行动物中分析前脑细胞类型的转录组差异.
主要方法:
- 分析了来自小鼠,人类,歌鸟和乌的七个单细胞和单核RNA测序数据集.
- 转录组主要成分 (PCs) 和细胞类型丰度的比较分析.
- 对胆固醇受体表达和电路建模的检查.
主要成果:
- 转录组PC显示了物种之间的显著差异,随着进化距离的增加而增加.
- 差异源于同源细胞类型内的基因表达分歧和细胞类型丰度的变化.
- 一些与小鼠内部神经元状态调节相关的胆固醇受体在物种之间表达不同.
结论:
- 转录组和状态依赖性处理之间的关系在物种之间并未完全保存.
- 基因表达和细胞类型组成的进化差异是物种特有的功能差异的基础.
- 胆固醇受体的不同表达可能会导致不同物种大脑状态的功能变化.
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