在低盐度下,呼吸链中的电子转移
Ana Paula Lobez1, Fei Wu1, Justin M Di Trani2,3
1Department of Biochemistry and Biophysics, The Arrhenius Laboratories for Natural Sciences, Stockholm University, Stockholm, Sweden.
Nature communications
|September 19, 2024
概括
静电相互作用是细胞呼吸的关键. 多个细胞染色体c分子与超复合体III2-IV结合,通过结构化的CIII2循环促进结合,从而实现高效的电子转移.
科学领域:
- 线粒体呼吸 线粒体呼吸
- 蛋白质与蛋白质的相互作用
- 生物物理学的生物物理.
背景情况:
- 细胞静电相互作用在生物过程中起着至关重要的作用.
- 细胞染色体c (cyt. c) 是线粒体呼吸链中的关键电子载体.
- 超级复合体III2-IV是电子传输链的主要组成部分.
研究的目的:
- 为了研究细胞之间的静电相互作用. c 和来自Saccharomyces cerevisiae的超级复合体III2-IV.
- 阐明低盐度电子转移的结构基础.
主要方法:
- 低温电子显微镜 (cryo-EM) 在2.4 Å分辨率.
- 稳定状态运动研究.
- 分析蛋白质结构和分子相互作用.
主要成果:
- 多个细胞组合. c分子与超复杂III2-IV表面结合,这是动力学研究 (希尔系数≥2) 和冷EM数据表明的.
- 负电荷的CIII2子单元 (Qcr6,Qcr9) 的循环结构与细胞相互作用. 在C.C.C.中,你会发现
- 在更高分辨率下,CIV质子通路中的水分子和心血管蛋白分子被识别出来.
结论:
- 降低静电选可以促进多个细胞的参与. c 分子. c 分子.
- 静电结构的CIII2循环可以直接引进细胞. 在CIII2和CIV之间进行高效的电子转移.
- 这项研究为线粒体电子运输机制提供了新的见解.
相关概念视频
The Electron Transport Chain
16.3K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
16.3K
The Supercomplexes in the Crista Membrane
2.5K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
2.5K
Electron Transport Chains
97.6K
The final stage of cellular respiration is oxidative phosphorylation that consists of two steps: the electron transport chain and chemiosmosis. The electron transport chain is a set of proteins found in the inner mitochondrial membrane in eukaryotic cells. Its primary function is to establish a proton gradient that can be used during chemiosmosis to produce ATP and generate electron carriers, such as NAD+ and FAD, that are used in glycolysis and the citric acid cycle.
The ETC is comprised of...
The ETC is comprised of...
97.6K
Electron Transport Chain: Complex III and IV
7.2K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
7.2K
Electron Transport Chain: Complex I and II
12.2K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
12.2K
Chemiosmosis
97.3K
Oxidative phosphorylation is a highly efficient process that generates large amounts of adenosine triphosphate (ATP), the basic unit of energy that drives many cellular processes. Oxidative phosphorylation involves two processes— the electron transport chain and chemiosmosis.
Electron Transport Chain
The electron transport chain involves a series of protein complexes on the inner mitochondrial membrane that undergo a series of redox reactions. At the end of this chain, the electrons...
Electron Transport Chain
The electron transport chain involves a series of protein complexes on the inner mitochondrial membrane that undergo a series of redox reactions. At the end of this chain, the electrons...
97.3K


