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一种新的液体血衍生物通过对Nrf2的上调抑制了黑色素生成

Ju Hyun Yun¹, Yeon Soo Kim², Hee Young Kang³

¹Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Ewha Womans University, Seoul, 07985, Korea.

Scientific reports

|September 19, 2024

概括

液体血通过抑制人类黑色素细胞中的黑色素合成,有效地减少色素. 这种新的抗黑色素生成方法针对关键路径,为治疗色素系疾病提供了潜力.

科学领域:

生物医学工程生物医学工程
皮肤病学皮肤病学
等离子体物理学的物理学

背景情况:

非热等离子体 (NTP) 是一个有前途的生物医学技术.
它在治疗异常色素的应用正在出现.
血诱导的抗黑色素生成的精确机制仍未得到充分研究.

研究的目的:

为了研究液体血的抗黑色素效应.
阐明参与血介导抑制黑色素合成的分子途径.
评估液体血作为一种潜在的治疗颜色系疾病的药物.

主要方法:

在培养介质中使用NTP喷射器与和氧生成液态血.
在斑马鱼和人类初级黑色素细胞中评估了抗黑色素效应.
基因和蛋白质表达 (MITF,铁酶,Wnt/β-catenin通路) 通过西部涂抹,RT-PCR和免疫组织化学分析.
测量了细胞内活性氧物种 (ROS) 和Nrf2水平.

主要成果:

液体血显著降低了斑马鱼的色素,并抑制了人类黑色素细胞中的黑色素合成.
治疗导致细胞内ROS降低和Nrf2表达增加.
液体血下调了关键的黑色素发生调节剂,包括MITF和铁酶.
抑制了Wnt/β-catenin通路,抑制了β-catenin的核转位和随后的黑色素生成.

结论:

液体血显示出强大的抗黑色素原特性.
该机制涉及通过Wnt/β-catenin通路向下调节MITF和铁酶.
液体血显示出作为一种新的色素系疾病治疗策略的潜力.

关键词:

美国MITF MITF 在β-catenin中,液体类型的非热等离子体 (液体等离子体)黑色素是黑色素的一种.黑色素细胞黑色素细胞

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